Insect hypertrehalosemic hormone: isolation and primary structure from Blaberus discoidalis cockroaches.
Insect hypertrehalosemic hormone: isolation and primary structure from Blaberus discoidalis cockroaches.
复制标题
昆虫高海藻糖激素：Blaberus Discoidalis 蟑螂的分离和初级结构。
DOI：
10.1016/0006-291x(86)90784-9
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发表时间：
1986
影响因子：
3.1
通讯作者：
Knight,DW
中科院分区：
文献类型：
--
作者：
Hayes,TK;Keeley,LL;Knight,DW
一种新的神经激素被分离出来，其结构特征是提高了蟑螂的血淋巴碳水化合物(海藻糖)水平。采用高效液相快速分离的方法，高产率地分离了该激素。序列为pGlu-Val-Asn-Phe-Ser-Pro-Gly-Trp-Gly-Thr-NH2。合成了所建议的序列，证实了其结构。合成的多肽具有与天然多肽相同的层析和生物学性质。
A new neurohormone was isolated and structurally characterized that increased hemolymph carbohydrate (trehalose) levels in the cockroach, Blaberus discoidalis. The hormone was isolated in high yield by a rapid HPLC procedure. The sequence, pGlu-Val-Asn-Phe-Ser-Pro-Gly-Trp-Gly-Thr-NH 2, was suggested from gas-phase Edman degradation of a peptide fragment of the natural peptide after deblocking with pyroglutamate aminopeptidase. The structure was confirmed by synthesis of the suggested sequence. The synthetic peptide had identical chromatographic and biological properties as the natural peptide.