Comprehensive approach to genes involved in cell wall modifications in Arabidopsis thaliana
Comprehensive approach to genes involved in cell wall modifications in Arabidopsis thaliana
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DOI：
10.1007/s11103-005-5344-7
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发表时间：
2005-05-01
影响因子：
5.1
通讯作者：
Nishitani, K
中科院分区：
文献类型：
--
作者：
Imoto, K;Yokoyama, R;Nishitani, K
植物细胞壁具有超分子结构，由多种非均相聚合物组成。几千种酶和结构蛋白直接参与拟南芥动态结构的构建过程和功能方面。这些蛋白质大多是由多基因家族编码的，每个家族中的大多数成员在结构特征上具有显著的相似性，但往往表现出不同的表达谱和生理功能。因此，对于细胞壁动力学的分子解剖，有必要区分蛋白质家族中的单个成员。作为表征细胞壁动力学过程的第一步，我们制造了一个基因特异性70-mer寡核苷酸微阵列，该微阵列由765个基因组成，分为30个假定的与拟南芥细胞壁动力学有关的蛋白质家族。通过使用该阵列系统，我们确定了几组表现出器官优先表达谱的基因。我们还确定了在拟南芥花序茎的某些特定生长阶段表达差异的基因组。我们的研究结果表明，在每个假定的细胞壁相关基因家族中，家族成员之间存在角色划分。
The plant cell wall is of supermolecular architecture, and is composed of various types of heterogeneous polymers. A few thousand enzymes and structural proteins are directly involved in the construction processes, and in the functional aspects of the dynamic architecture in Arabidopsis thaliana. Most of these proteins are encoded by multigene families, and most members within each family share significant similarities in structural features, but often exhibit differing expression profiles and physiological functions. Thus, for the molecular dissection of cell wall dynamics, it is necessary to distinguish individual members within a family of proteins. As a first step towards characterizing the processes involved in cell wall dynamics, we have manufactured a gene-specific 70-mer oligo microarray that consists of 765 genes classified into 30 putative families of proteins that are implicated in the cell wall dynamics of Arabidopsis. By using this array system, we identified several sets of genes that exhibit organ preferential expression profiles. We also identified gene sets that are expressed differentially at certain specific growth stages of the Arabidopsis inflorescence stem. Our results indicate that there is a division of roles among family members within each of the putative cell wall-related gene families.