Hybrid hydrogels self-assembled from HPMA copolymers containing peptide grafts.
Hybrid hydrogels self-assembled from HPMA copolymers containing peptide grafts.
复制标题
由含有肽接枝物的 HPMA 共聚物自组装而成的混合水凝胶。
DOI：
10.1002/mabi.200500208
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发表时间：
2006
影响因子：
4.6
通讯作者：
Kopecek,Jindrich
中科院分区：
文献类型：
--
作者：
Yang,Jiyuan;Xu,Chunyu;Kopecková,Pavla;Kopecek,Jindrich
总结：接枝共聚物被设计成通过卷曲螺旋肽结构域的相互作用介导自组装成水凝胶。选择HPMA的线性亲水性聚合物作为骨架，并且共价连接到骨架的卷曲螺旋形成肽形成移植物。使用微观流变学来评估接枝共聚物到水凝胶中的自组装。结果表明，每条链的卷曲螺旋接枝物的长度和数量对凝胶化过程具有显著影响。需要至少4个七单元体来实现接枝共聚物缔合成水凝胶。含有5个七肽接枝物的共聚物的CD光谱进一步表明卷曲螺旋的形成可能有助于自组装。含有CC4肽的接枝共聚物的接枝率表明，每个大分子需要阈值量的接枝物以形成三维结构。这些研究证明了接枝共聚物产生具有所需和可控结构的自组装水凝胶的潜力。
Summary:Graft copolymers were designed that self‐assemble into hydrogels mediated by the interaction of coiled‐coil peptide domains. A linear hydrophilic polymer of HPMA was chosen as the backbone, and coiled‐coil forming peptides, covalently attached to the backbone, formed the grafts. Microrheology was used to evaluate the self‐assembly of graft copolymers into hydrogels. The results revealed that the length and the number of coiled‐coil grafts per chain had a significant influence on the gelation process. At least 4 heptads were needed to achieve the association of graft copolymers into hydrogels. CD spectra of the copolymer containing 5 heptad grafts further suggested that coiled‐coil formation may contribute to the self‐assembly. Gelation of graft copolymers containing CC4 peptides indicated that a threshold amount of grafts per macromolecule is needed to form a three‐dimensional structure. These studies demonstrated a potential of the graft copolymers to create self‐assembling hydrogels with desirable and controllable structures.