Suppression of red light-induced resistance in broad beans to Botrytis cinerea by salicylic acid
Suppression of red light-induced resistance in broad beans to Botrytis cinerea by salicylic acid
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DOI：
10.1016/j.pmpp.2005.03.006
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发表时间：
2005-01-01
影响因子：
2.7
通讯作者：
Arase, S
中科院分区：
文献类型：
--
作者：
Khanam, NN;Ueno, M;Arase, S
研究了水杨酸（SA）对红外光诱导下蚕豆对灰霉病（Botrytis cinerea）抗性的影响。在红光处理下，蚕豆叶片的病变形成和真菌发育均受到抑制，产生抗真菌化合物。然而，SA预处理可以剂量依赖性地抑制红光诱导抗性的表达，产生过氧化氢（H2O2）。在H2O2清除剂抗坏血酸或NADPH氧化酶抑制剂二苯碘的存在下，即使在sa预处理的蚕豆叶片中，红光诱导的抗性也能恢复。这些结果表明，红光诱导的蚕豆对绿芽孢杆菌抗性的破坏是由膜介导的H2O2生成诱导的。另一方面，在红光处理下，蚕豆叶片过氧化氢酶活性显著增强，而SA和氨三唑处理的叶片过氧化氢酶活性不显著增强。我们假设抗氧化酶过氧化氢酶活性的增强有助于抑制蚕豆细胞死亡，清除由灰孢杆菌感染产生的内源性H2O2，并促进活宿主细胞依赖于激发剂产生抗真菌成分；因此，红光诱导的电阻可能会建立起来。蚕豆中sa依赖的信号通路可能在植物-病原体病理系统中起着不同的作用。(c) 2005 Elsevier Ltd版权所有。
We investigated the effect of salicylic acid (SA) on red light-induced resistance in broad beans to Botrytis cinerea. Both lesion formation and fungal development were suppressed on broad bean leaves kept under red light, producing anti-fungal compound(s). However, SA pretreatment inhibited expression of red light-induced resistance dose-dependently, generating hydrogen peroxide (H2O2). Red light-induced resistance was recovered in the presence of a H2O2 scavenger, ascorbic acid or a NADPH oxidase inhibitor, diphenylene iodonium even in SA-pre-treated broad bean leaves. These results suggest that breakdown of red light-induced resistance in broad beans to B. cinerea is induced by membrane-mediated H2O2 generation. On the other hand, catalase activity in broad bean leaves was significantly enhanced under red light, but not in those pre-treated with SA and aminotriazole. We hypothesize that enhanced antioxidant enzyme catalase activity contributes to the inhibition of cell death in broad beans scavenging endogenous H2O2 generated by B. cinerea infection and to elicitor-dependent production of anti-fungal component(s) by living host cells; as a consequence, red light-induced resistance may be established. It is possible that an SA-dependent signaling pathway in broad beans is playing different roles in the plant-pathogen pathosystem. (c) 2005 Elsevier Ltd. All rights reserved.