KIT (CDII7) is frequently overexpressed in thymic carcinomas but is absent in thymomas
KIT (CDII7) is frequently overexpressed in thymic carcinomas but is absent in thymomas
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DOI：
10.1002/path.1514
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发表时间：
2004-03-01
影响因子：
7.3
通讯作者：
Chiang, H
中科院分区：
文献类型：
--
作者：
Pan, CC;Chen, PCH;Chiang, H
KIT (CD117) 是一种酪氨酸激酶受体，尚未在上皮肿瘤中得到广泛研究。对 671 例组织芯片上的 KIT 表达进行系统免疫组织化学调查，发现胸腺癌频繁表达 KIT。取出 22 例胸腺癌、110 例胸腺瘤和 16 例非肿瘤性胸腺进行进一步分析。免疫组织化学显示，19 例 (86%) 胸腺癌显示异质性至弥漫性膜阳性，而胸腺瘤或正常胸腺均未含有阳性上皮细胞。使用逆转录聚合酶链式反应 (RT-PCR)，可以在所有免疫组织化学阳性病例中证明 c-kit 转录本。还对胸腺癌进行了 c-kit 近膜结构域（外显子 9 和 11）和酪氨酸激酶结构域（外显子 13 和 17）的 PCR 扩增和直接测序，但未发现突变。一些非胸腺上皮肿瘤显示频繁的 KIT 表达，包括唾液腺腺样囊性癌（100% 阳性）、嫌色肾细胞癌（94%）、肾嗜酸细胞瘤（67%）和神经内分泌肿瘤（34%）。其他癌症很少对 KIT 产生免疫反应。本研究结果表明 KIT 参与胸腺癌的发病机制。胸腺癌中 KIT 的过度表达具有潜在的诊断用途，可将这些肿瘤与胸腺瘤和其他部位产生的癌（这些部位很少表达 KIT）区分开来。版权所有 (C) 2004 大不列颠及爱尔兰病理学会。约翰·威利儿子有限公司出版
KIT (CD117), a tyrosine kinase receptor, has not been widely studied in epithelial tumours. In a systematic immunohistochemical survey of KIT expression on tissue arrays incorporating 671 cases, it was found that thymic carcinomas frequently express KIT. Twenty-two thymic carcinomas, 110 thymomas, and 16 non-neoplastic thymus glands were retrieved for further analyses. Immunohistochemically, 19 (86%) thymic carcinomas revealed heterogeneous to diffuse membranous positivity, whereas no thymomas or normal thymus glands contained positive epithelial cells. Using reverse transcriptase-polymerase chain reaction (RT-PCR), c-kit transcripts could be demonstrated in all immunohistochemically positive cases. PCR amplification and direct sequencing of the c-kit juxtamembrane domains (exons 9 and 11) and tyrosine kinase domain (exons 13 and 17) were also performed on the thymic carcinomas but mutations were not found. Some non-thymic epithelial tumours showed frequent KIT expression including adenoid cystic carcinomas of the salivary gland (100% positive), chromophobe renal cell carcinomas (94%), renal oncocytomas (67%), and neuroendocrine tumours (34%). Other carcinomas were infrequently immunoreactive for KIT. The findings of this study suggest that KIT is involved in the pathogenesis of thymic carcinomas. The overexpression of KIT in thymic carcinomas has potential diagnostic utility in differentiating these tumours from thymomas and carcinomas arising from other sites, which express KIT infrequently. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd.