Regulating bacterial transcription with small RNAs.
Regulating bacterial transcription with small RNAs.
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用小 RNA 调节细菌转录。
DOI：
10.1101/sqb.2006.71.033
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Wassarman,KM
中科院分区：
文献类型：
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作者：
Storz,G;Opdyke,JA;Wassarman,KM
近年来，计算机和分子生物学方法的结合已经导致越来越多的细菌及其质粒和质粒编码的小的非编码RNA的鉴定。大多数小分子RNA在转录后水平上起作用，通过与靶mRNA的5′端碱基配对来调节mRNA的稳定性或翻译。然而，已经发现小RNA的子集调节转录。一个例子是丰富的6S RNA，它被认为通过模仿启动子DNA的开放构象来竞争RNA聚合酶的DNA结合。其他小RNA通过与mRNA序列的碱基配对相互作用影响转录终止。在这里，我们讨论了目前的理解和问题的作用，小RNA在调节转录。
In recent years, the combinations of computational and molecular approaches have led to the identification of an increasing numberof small, noncoding RNAs encoded by bacteria and their plasmids and phages. Most of the characterized small RNAs havebeen shown to operate at a posttranscriptional level, modulating mRNA stability or translation by base-pairing with the 5′ regionsof the target mRNAs. However, a subset of small RNAs has been found to regulate transcription. One example is the abundant6S RNA that has been proposed to compete for DNA binding of RNA polymerase by mimicking the open conformation of promoterDNA. Other small RNAs affect transcription termination via base-pairing interactions with sequences in the mRNA. Here, we discuss current understanding and questions regarding the roles of small RNAs in regulating transcription.