Prevalence of High-Level Aminoglycoside Resistance and Genes Encoding Aminoglycoside-Modifying Enzymes in Enterococcus faecalis and Enterococcus faecium Isolated in a University Hospital in Tokyo
Prevalence of High-Level Aminoglycoside Resistance and Genes Encoding Aminoglycoside-Modifying Enzymes in Enterococcus faecalis and Enterococcus faecium Isolated in a University Hospital in Tokyo
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DOI：
10.7883/yoken.jjid.2019.416
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发表时间：
2020-11-01
影响因子：
2.2
通讯作者：
Tateda, Kazuhiro
中科院分区：
文献类型：
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作者：
Harada, Sohei;Shibue, Yasushi;Tateda, Kazuhiro
高水平氨基糖苷类耐药性（HLAR）限制了侵袭性肠球菌感染的治疗选择。我们分别使用纸片扩散法、聚合酶链式反应和基于硝基头孢菌素的测试，检查了 2010 年从东京大学医院患者体内分离出的粪肠球菌和屎肠球菌中 HLAR 的流行情况、编码氨基糖苷修饰酶的基因的携带情况以及 β-内酰胺酶的产生情况。在分析的 100 株粪肠球菌分离株中，有 30 株具有高水平耐药性(HLR) 对庆大霉素，22 个分离株对链霉素具有 HLR。在分析的 40 株屎肠球菌分离株中，9 株分离株具有庆大霉素 HLR，9 株分离株具有链霉素 HLR。在 39 个庆大霉素-HLR 肠球菌分离株中，24 个分离株对链霉素不具有 HLR。所有 39 个具有庆大霉素 HLR 的分离株以及 101 个不具有 HLR 的分离株中的 19 个均携带 aac(6')-Ie-aph(2'')-Ia。 31 株链霉素-HLR 分离株中的 25 株证实携带 ant(6)-Ia。在粪肠球菌和屎肠球菌分离株中均未记录到β-内酰胺酶的产生。全基因组测序分析显示，除了一种携带 aac(6)-Ie-aph(2 '')-Ia 和 ant(6)-Ia 的粪肠球菌分离株外，所有菌株均属于序列类型 (ST) 4 (n = 8)、ST16 (n = 4) 或 ST179 (n = 9)。然而，即使在同一 ST 的分离株中，大多数分离株对也具有 > 10 个单核苷酸多态性。
High-level aminoglycoside resistance (HLAR) limits treatment options for invasive enterococcal infections. We examined the prevalence of HLAR, carriage of genes encoding aminoglycoside-modifying enzymes, and production of beta-lactamase using the disk diffusion method, polymerase chain reaction, and a nitrocefin-based test, respectively, in Enterococcus faecalis and Enterococcus faecium isolated from patients at a university hospital in Tokyo in 2010. Of the 100 E. faecalis isolates analyzed, 30 isolates had high-level resistance (HLR) to gentamicin, and 22 isolates had HLR to streptomycin. Of the 40 E. faecium isolates analyzed, 9 isolates had HLR to gentamicin, and 9 isolates had HLR to streptomycin. Of the 39 gentamicin-HLR enterococcal isolates, 24 isolates were non-HLR to streptomycin. All 39 isolates with HLR to gentamicin as well as 19 of 101 without HLR carried aac(6')-Ie-aph(2 '')-Ia. Carriage of ant(6)-Ia was confirmed in 25 of 31 streptomycin-HLR isolates. Production of P-lactamase was documented in none of the E. faecalis and E. faecium isolates. Whole-genome sequencing analysis revealed that all but one E. faecalis isolate that carried aac(6)-Ie-aph(2 '')-Ia and ant(6)-Ia belonged to sequence type (ST) 4 (n = 8), ST16 (n = 4), or ST179 (n = 9). Nevertheless, most of the pairs of isolates had > 10 single-nucleotide polymorphisms even among the isolates of the same ST.