Evidence for mating of the "asexual" yeast Candida albicans in a mammalian host
Evidence for mating of the "asexual" yeast Candida albicans in a mammalian host
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DOI：
10.1126/science.289.5477.307
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发表时间：
2000-07-14
期刊：
影响因子：
56.9
通讯作者：
Johnson, AD
中科院分区：
文献类型：
--
作者：
Hull, CM;Raisner, RM;Johnson, AD
自近80年前分类以来，人类病原体白色念珠菌一直被指定为无性酵母。本文介绍了C.在交配型样（MTL）基因座（一组类似于其他真菌交配型基因座的基因）发生细微改变的白色念珠菌菌株。这些衍生物在接种到哺乳动物宿主中后能够交配。C.白色念珠菌是二倍体生物体，但从小鼠宿主中分离的大多数交配产物对于可以严格监测的两条染色体是四体的，并且总体上表现出显著高于2n的DNA含量。这些观察结果表明，白色念珠菌可以有性重组。
Since its classification nearly 80 years ago, the human pathogen Candida albicans has been designated as an asexual yeast. In this report, we describe the construction of C. albicans strains that were subtly altered at the mating-type-like (MTL) locus, a cluster of genes that resembles the mating-type Loci of other fungi. These derivatives were capable of mating after inoculation into a mammalian host. C. albicans is a diploid organism, but most of the mating products isolated from a mouse host were tetrasomic for the two chromosomes that could be rigorously monitored and, overall, exhibited substantially higher than 2n DNA content. These observations demonstrated that C, albicans can recombine sexually.