Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots.
Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots.
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通过非放射性 Northern 印迹检测长非编码 RNA 表达。
DOI：
10.1007/978-1-4939-3378-5_14
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Zhang,Lin
中科院分区：
文献类型：
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作者：
Hu,Xiaowen;Feng,Yi;Hu,Zhongyi;Zhang,Youyou;Yuan,Chao-Xing;Xu,Xiaowei;Zhang,Lin
随着测序技术和转录组分析的进步，估计高达75%的人类基因组被转录为RNA。这一发现促使人们对非编码 RNA 的生物学功能进行深入研究，并导致 microRNA 作为疾病发病机制和治疗应用中重要参与者的非常令人兴奋的发现。长非编码 RNA (lncRNA) 的研究还处于起步阶段，但 lncRNA 具有广泛的生物调控作用。作为一类新型的RNA转录本，lncRNA的表达水平和剪接变异多种多样。 Northern blot分析可以帮助我们了解lncRNA的身份、大小和丰度。在这里，我们描述如何使用 Northern blot 来确定 lncRNA 丰度并识别给定 lncRNA 的不同剪接变体。
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75 % of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. As a novel class of RNA transcripts, the expression level and splicing variants of lncRNAs are various. Northern blot analysis can help us learn about the identity, size, and abundance of lncRNAs. Here we describe how to use northern blot to determine lncRNA abundance and identify different splicing variants of a given lncRNA.