Towards quantitative analysis of enamel erosion by focused ion beam tomography
Towards quantitative analysis of enamel erosion by focused ion beam tomography
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DOI：
10.1016/j.dental.2018.08.304
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发表时间：
2018-11-01
期刊：
影响因子：
5
通讯作者：
Chevalier, Jerome
中科院分区：
文献类型：
--
作者：
Cougot, Nancie;Douillard, Thierry;Chevalier, Jerome
目标.这项工作的目的是一个概念的证明，引入一个新的定量的三维分析的牙齿侵蚀获得的聚焦离子束（FIB）断层扫描与银硝酸盐渗透到孔隙中的蚀刻牙釉质。选择的一个样品是去除无泪表面后的健康釉质。另一种是在用正磷酸进行额外的攻击后进行研究。两个表面都通过浸渍用硝酸银渗透。脱水后，在双柱FIB/SEM站中观察样品。连续FIB切片进行与3 nA的电流在30 keV和20 nm的增量步长为健康的牙釉质和40 nm的蚀刻一个。利用Fiji软件和BoneJ插件进行三维分析，获得表征组织的几个参数：非矿化相含量（NMP）、连通孔隙率（CPF）和NMP的各向异性程度（DA）。健康牙釉质显示NMP含量为0.5体积%，在棱柱内部和棱柱之间具有非矿化区域的双峰分布。在健康的牙釉质中没有观察到银渗透，表明不存在开放孔隙。相反，在酸暴露后观察到银银渗透，直到12 μ m的深度，这允许计算3.1体积%的互连孔隙体积分数（CPF），主要是在棱镜之间。DA值为0.56的健康釉质和0.81的酸蚀表面被确定，突出了更高程度的各向异性在后者。使用NMP、CPF和DA进行FIB断层扫描的定量分析应有助于更好地理解和随访牙齿侵蚀、侵蚀与磨损或磨损过程之间的相关性以及开发釉质再矿化程序的能力。(C)2018年牙科材料学院。爱思唯尔公司出版All rights reserved.
Objectives. The purpose of this work is a proof of concept to introduce a new quantitative 3D-analysis of dental erosion obtained by focused ion beam (FIB) tomography associated with silver nitrate penetration into porosities in etched enamel.Methods. One sample selected was sound enamel after removal of the aprismatic surface. The other was studied after applying an additional attack with orthophosphoric acid. Both surfaces were infiltrated with silver nitrate via immersion. After dehydration, samples were observed in a dual column FIB/SEM station. Serial FIB sectioning was conducted with a current of 3 nA at 30 keV and an increment step of 20 nm for the healthy enamel and of 40 nm for the etched one. 3D analysis was performed with Fiji software and BoneJ plugin and several parameters were obtained to characterize the tissue: non-mineralized phase content (NMP), connected porosity fraction (CPF) and degree of anisotropy (DA) of the NMP.Results. Healthy enamel showed an NMP content of 0.5 vol.%, with a bimodal distribution of non-mineralized regions, inside the prisms and between the prisms. No silver penetration was noticed in the healthy enamel, demonstrating the absence of open porosity. In contrast, silver nitrate penetration after acidic exposure was observed, up to a depth of 12 mu m, which allowed the calculation of an interconnected porosity volume fraction (CPF) of 3.1 vol.%, mostly between the prisms. Values for DA of 0.56 for sound enamel and 0.81 for acid-etched surface were determined, highlighting a higher degree of anisotropy in the latter.Significance. Quantitative analysis of FIB tomography using NMP, CPF and DA should contribute to a better understanding and follow up of dental erosion, correlation between erosion and attrition or abrasion process, and the ability to develop enamel remineralization procedures. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.