Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
复制标题
DOI：
10.1016/j.archoralbio.2010.08.002
复制
发表时间：
2010-12-01
影响因子：
3
通讯作者：
Nogueira-Filho, Getulio
中科院分区：
文献类型：
--
作者：
Goncalves, Thais Oliveira;Costa, Dirceu;Nogueira-Filho, Getulio
目的：牙周医学的出现增加了人们对牙周炎患者外周血细胞行为的兴趣。本研究的目的是评估白细胞介素（IL）-8，肿瘤坏死因子-α（TNF-α）通过从慢性牙周炎受试者的外周血获得的大肠杆菌脂多糖（LPS）刺激的外周血单核细胞（PBMC）释放的TNF-α、IL-6和IL-10。PBMC样品分离自19个全身健康的供体，分为全身性慢性牙周炎（n = 10）和健康（n = 9）受试者。将细胞在含有RPM！1640，并用1.0 ng/mL E. coli LPS。结果：牙周炎患者外周血单个核细胞分泌的TNF-α和IL-6水平明显高于健康对照组（P < 0.05）;健康人PBMC培养上清中IL-8含量高于牙周炎患者（P < 0.05）。结论：本研究结果表明，E. coli LPS刺激的牙周炎受试者PBMC与健康受试者PBMC相比，呈现不同的细胞因子释放模式。这种现象可能会影响局部牙周炎以及全身性疾病。(C)2010爱思唯尔有限公司版权所有。
Objective: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factor-alpha (TNF-alpha), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects.Design: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated for 24-48 h in 500 mu L wells containing RPM! 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-alpha, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA).Results: PBMC cells from periodontitis subjects released higher levels of TNF-alpha and IL-6 than those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between groups.Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases. (C) 2010 Elsevier Ltd. All rights reserved.