AN IMMUNOHISTOCHEMICAL QUANTIFICATION OF FIBROUS ASTROCYTES IN THE AGING HUMAN CEREBRAL-CORTEX
AN IMMUNOHISTOCHEMICAL QUANTIFICATION OF FIBROUS ASTROCYTES IN THE AGING HUMAN CEREBRAL-CORTEX
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DOI：
10.1016/0197-4580(87)90051-0
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发表时间：
1987-01-01
影响因子：
4.2
通讯作者：
TERRY, RD
中科院分区：
文献类型：
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作者：
HANSEN, LA;ARMSTRONG, DM;TERRY, RD
为了确定皮质纤维星形胶质细胞是否随着年龄的增长而增加，我们研究了25名年龄在24岁到100岁之间，没有痴呆或其他大脑疾病的临床或病理证据的患者。取中额叶皮层石蜡切片，采用胶质中间丝蛋白亲和素免疫标记法进行染色。免疫标记的纤维星形胶质细胞在分子层和细胞层（皮质层2-6）中计数。两层纤维星形胶质细胞的数量在个体之间差异很大，在分子层中，它们的数量与年龄的增长没有显著相关。然而，在细胞层，尽管在相同的几十年里，个体之间的细胞数量相差很大，但随着年龄的增长，细胞数量呈显著的线性增长。我们的数据表明，在70岁之后，这种增加发生或显著加速，但病例数排除了得出这样的结论与统计信心。然而，当将患者分为70岁以下和老年人时，后者的细胞层纤维状星形胶质细胞比前者明显增加。
In order to determine whether cortical fibrous astrocytes increase with age, we studied 25 patients ranging in age from 24 to 100 years with no clinical or pathological evidence of dementia or other cerebral disorder. Paraffin sections of mid-frontal cortex were obtained and stained with the avidin-biotin immunolabeling procedure for glial intermediate filament protein. The resulting immunolabeled fibrous astrocytes were then counted in the molecular and cellular (cortical laminae 2-6) layers. Populations of fibrous astrocytes in both layers varied widely among individuals, and in the molecular layer their numbers were not significantly correlated with advancing age. In the cellular layer, however, despite widely ranging cell counts among individuals within the same decades of life, there was a significant linear increase with age. Our data suggest that the increase occurs or accelerates significantly after age 70, but the case numbers preclude reaching such a conclusion with statistical confidence. However, when the patients are divided into those less than 70 and those older, fibrous astrocytes in the cellular layer are shown to be significantly increased in the latter group compared to the former.