Identification and characterization of a novel gene-encoded antioxidant peptide obtained from amphibian skin secretions.
Identification and characterization of a novel gene-encoded antioxidant peptide obtained from amphibian skin secretions.
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从两栖动物皮肤分泌物中获得的新型基因编码抗氧化肽的鉴定和表征。
DOI：
10.1080/14786419.2018.1499635
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发表时间：
2020
影响因子：
2.2
通讯作者：
Yang Xinwang
中科院分区：
文献类型：
--
作者：
Wang Ying;Cao Xiaoqing;Fu Zhe;Wang Siyuan;Li Xiaojie;Liu Naixin;Feng Zhuo;Yang Meifeng;Tang Jing;Yang Xinwang
两栖动物皮肤分泌的小分子抗氧化肽在宿主防御中起重要作用。然而，对这些肽的识别仍处于起步阶段。在这里，我们发现了一种新的基因编码的抗氧化肽（命名为OM-GF 17）从两栖动物的皮肤分泌物，臭蛙margaretae。OM-GF 17是由61个氨基酸残基的前肽经翻译后加工而成，其氨基酸序列为“GFFKWHPRCGEEHSMWT”，分子量为2135.7 Da。功能分析表明，OM-GF 17清除ABTS+，DPPH，NO和减少铁氧化。我们的研究结果还表明，5个氨基酸残基，包括Cys，Pro，Met，Trp和Phe，与OM-GF 17的抗氧化活性有关。此外，0 M-GF 17没有表现出直接的微生物杀灭活性。这种新的基因编码的抗氧化肽可能有助于开发新的抗氧化剂，并增加我们对两栖动物皮肤生物功能的理解。
Amphibian skin is known to secrete gene-encoded antioxidant peptides of small molecular weight, which play important roles in host defense. However, recognition of such peptides is still in its infancy. Here, we discovered a novel gene-encoded antioxidant peptide (named OM-GF17) from skin secretions of amphibian species,Odorrana margaretae. Produced by the post-translational processing of a 61-residue prepropeptide, the amino acid sequence of OM-GF17 was 'GFFKWHPRCGEEHSMWT', with a molecular mass of 2135.7 Da. Functional analysis revealed that OM-GF17 scavenged ABTS+, DPPH, NO and decreased iron oxidation. Our results also implied that five amino acid residues, including Cys, Pro, Met, Trp, and Phe, be related to the antioxidant activity of OM-GF17. Furthermore, OM-GF17 did not exhibit direct microbe-killing activity. This novel gene-encoded antioxidant peptide could help in the development of new antioxidant agents and increase our understanding of the biological functions of amphibian skin.