Separase, polo kinase, the kinetochore protein Slk19 and Spo12 function in a network that controls Cdc14 localization during early anaphase
Separase, polo kinase, the kinetochore protein Slk19 and Spo12 function in a network that controls Cdc14 localization during early anaphase
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DOI：
10.1016/s0092-8674(02)00618-9
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发表时间：
2002-01-25
期刊：
影响因子：
64.5
通讯作者：
Amon, A
中科院分区：
文献类型：
--
作者：
Stegmeier, F;Visintin, R;Amon, A
在芽生酵母中，磷酸酶CDC14是退出有丝分裂的关键调节因子，在后期从核仁中的Cfi1/Net1中释放出来。一个被称为有丝分裂退出网络(MEN)的信号级联控制着这种释放。我们已经确定了一个调控网络，FARE(CDC 14早期后期释放)网络，它促进了早期后期核仁中CDC14的释放。恐惧网络由Polo激酶CDG5、分离酶ESP1、动粒相关蛋白Slk19和Spo12组成。我们还表明，恐惧网络在早期后期启动了CDC14从CFI1/Net1的释放，而男性在后期保持了CDC14的释放状态。我们认为，恐惧网络释放的CDC14的一个功能是刺激男性的活动。
In budding yeast, the phosphatase Cdc14, a key regulator of exit from mitosis, is released from its inhibitor Cfi1/Net1 in the nucleolus during anaphase. A signaling cascade, known as the mitotic exit network (MEN), controls this release. We have identified a regulatory network, the FEAR (Cdc fourteen early anaphase release) network that promotes Cdc14 release from the nucleolus during early anaphase. The FEAR network is comprised of the polo kinase CdG5, the separase Esp1, the kinetochore-associated protein Slk19, and Spo12. We also show that the FEAR network initiates Cdc14 release from Cfi1/Net1 during early anaphase, and MEN maintains Cdc14 in the released state during late anaphase. We propose that one function of Cdc14 released by the FEAR network is to stimulate MEN activity.