Multiparameter resonant imaging for studying cell interactions.
Multiparameter resonant imaging for studying cell interactions.
复制标题
用于研究细胞相互作用的多参数共振成像。
DOI：
10.1038/s41377-018-0032-y
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Juan-Colás J
中科院分区：
文献类型：
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作者：
Juan-Colás J
光学显微镜技术的发展为从分子到细胞再到组织和器官的生命机制提供了重要的见解。荧光技术在这方面尤其成功，它阐明了生物途径，并帮助我们了解支撑所有生物的复杂关系。例如，1864年第一次使用荧光作为分析工具1为研究生物实体和相互作用铺平了道路，但直到近60年后的1930年代，荧光团才首次被用于对特定组织成分和不自体荧光的细菌进行生物调查2。这些进展导致了1941年第一次用可见标记标记抗体，这催生了免疫荧光领域，使我们对抗体结构有了前所未有的了解。然而，荧光技术需要添加标记，这使程序复杂化，并可能干扰我们试图了解的正是途径。5.因此，已经开发出新的方法，不需要添加荧光标记，而是使用光子共振来增强电磁场和感兴趣的生物对象之间的弱相互作用。此外，并行检测多个参数非常重要，因为有关生物体的密度、粘附性或运动性的信息以及生物对象的特性(如硬度和阻抗)都有助于我们对生物路径的理解。随着无标记共振显微镜技术的引入，朝着多参数表征这一目标迈出了重要的一步，该技术提供了对两者的深入了解
The development of optical microscopy techniques has provided major insights into the machinery of life, from molecules to cells to tissues and organs. Fluorescent techniques have been particularly successful in this respect by elucidating biological pathways and helping us understand the complex relationships that underpin all living organisms. For example, the first use of fluorescence as an analytical tool in 1864 1 paved the way for the study of biological entities and interactions, but it was not until nearly 60 years later, in the 1930s, that fluorophores were first employed to perform biological investigations into specific tissue components and bacteria that do not autofluoresce 2. These advances led to the first labeling of an antibody with a visible label in 1941 3, which gave birth to the field of immunofluorescence and allowed us obtain unprecedented insight into antibody structure.Nevertheless, fluorescence techniques require the addition of labels, which complicate the procedure and may interfere with the very pathways that we are trying to understand 4, 5. As a result, new methods have been developed that do not require the addition of fluorescent labels but use photonic resonances to enhance the weak interaction between electromagnetic fields and the biological objects of interest. Moreover, it is important to detect multiple parameters in parallel since information about the density, adhesion, or motility of a living body and properties such as the stiffness and impedance of biological objects all provide insights into our understanding of biological pathways. An important step toward this goal of multiparameter characterization has now been taken with the introduction of a resonant labelfree microscopy technique that offers insights into both