Detection of antibody to the foot-and-mouth disease virus (FMDV) non-structural polyprotein 3ABC in sheep by ELISA
Detection of antibody to the foot-and-mouth disease virus (FMDV) non-structural polyprotein 3ABC in sheep by ELISA
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DOI：
10.1016/j.jviromet.2005.01.012
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发表时间：
2005-05-01
影响因子：
3.1
通讯作者：
Paton, DJ
中科院分区：
文献类型：
--
作者：
Armstrong, RM;Cox, SJ;Paton, DJ
为评价检测口蹄疫病毒3ABC非结构蛋白免疫球蛋白的特异性和敏感性，建立了检测口蹄疫病毒3ABC非结构蛋白的新方法。气溶胶感染、气溶胶加直接接触感染和野外暴露的绵羊。尽管与结构蛋白抗体检测结果相比，暴露于口蹄疫的绵羊的田间血清呈阳性的较少，但所有12只在实验中感染而未接种疫苗的绵羊都在试验中血清转换。3ABC试验的特异性为98%或100%，以可疑范围内的血清反应为阳性或阴性为标准。然后，用该试验研究了接种口蹄疫疫苗的绵羊的抗体反应，并在4-14天后通过气溶胶攻击暴露于该病毒。不同动物的反应各不相同。无论是高剂量疫苗免疫还是低剂量疫苗免疫，3ABC抗体最有可能发生在攻毒后9天或以上的绵羊身上。非结构性反应在多次发生活口蹄疫病毒分离的动物中也更常见(可能更能表明真正的病毒复制)。(C)2005 Elsevier B.V.保留所有权利。
The specificity and sensitivity of an ELISA for detecting IgG to the 3ABC non-structural protein of foot-and-mouth disease (FMD) virus was evaluated in FMD naive. aerosol-infected, aerosol plus direct contact infected and field-exposed sheep. All 12 sheep that were experimentally infected without prior vaccination seroconverted in the test, although fewer field sera from FMD-exposed sheep were scored seropositive compared to test results for structural protein antibodies. The 3ABC test specificity was 98 or 100% according to whether sera reacting in the doubtful range were scored as positive or negative. The test was then used to investigate the antibody response of sheep vaccinated against FMD and exposed to the virus by an aerosol challenge 4-14 days later. The response of individual animals varied. Whether immunised with high or low doses of vaccine, the development of 3ABC antibody was most likely in sheep from which live virus was recovered at or beyond 9 days post-challenge. Non-structural responses were also more frequent in animals from which multiple incidences of live FMD virus isolation (perhaps more indicative of true virus replication) were demonstrated. (c) 2005 Elsevier B.V. All rights reserved.