Neuronal patterning by BMPs: a requirement for GDF7 in the generation of a discrete class of commissural interneurons in the mouse spinal cord
Neuronal patterning by BMPs: a requirement for GDF7 in the generation of a discrete class of commissural interneurons in the mouse spinal cord
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DOI：
10.1101/gad.12.21.3394
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发表时间：
1998-11-01
影响因子：
10.5
通讯作者：
Jessell, TM
中科院分区：
文献类型：
--
作者：
Lee, KJ;Mendelsohn, M;Jessell, TM
已知诱导因子指导脊椎动物中枢神经系统（CNS）的区域分化，但它们在个体神经元细胞类型的特化中的作用尚不清楚。我们已经研究了GDF 7的功能，BMP家族成员选择性表达的顶板细胞，在脊髓背侧神经元细胞类型的产生。我们发现GDF 7可以促进两种背感觉中间神经元D1 A和D1 B神经元的分化。在Gdf 7-null突变胚胎中，D1 A神经元的产生被消除，但D1 B神经元和其他识别的背侧中间神经元不受影响。这些发现表明，GDF 7是一个诱导信号，从顶板所需的规格的神经元身份在脊髓背侧和GDF 7和其他BMP家族成员表达的顶板在体内有非冗余的功能。更一般地说，这些结果表明，BMP信号可能有一个突出的作用，在哺乳动物中枢神经系统内的神经元身份的分配。
Inductive factors are known to direct the regional differentiation of the vertebrate central nervous system (CNS) but their role in the specification of individual neuronal cell types is less clear. We have examined the function of GDF7, a BMP family member expressed selectively by roof plate cells, in the generation of neuronal cell types in the dorsal spinal cord. We find that GDF7 can promote the differentiation in vitro of two dorsal sensory interneuron classes, D1A and D1B neurons. In Gdf7-null mutant embryos, the generation of D1A neurons is eliminated but D1B neurons and other identified dorsal interneurons are unaffected. These findings show that GDF7 is an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord and that GDF7 and other BMP family members expressed by the roof plate have non-redundant functions in vivo. More generally, these results suggest that BMP signaling may have a prominent role in the assignment of neuronal identity within the mammalian CNS.