Regulation of the chitin degradation and utilization system by the ChiX small RNA in Serratia marcescens 2170
Regulation of the chitin degradation and utilization system by the ChiX small RNA in Serratia marcescens 2170
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粘质沙雷氏菌 ChiX 小 RNA 调节几丁质降解和利用系统 2170
DOI：
10.1080/09168451.2015.1083399
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and T. Watanabe
中科院分区：
文献类型：
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作者：
K. Suzuki;M. Shimizu;N. Sasaki;C. Ogawa;H. Minami;H. Sugimoto;and T. Watanabe
粘质沙雷氏菌2170产生三种不同类型的几丁质酶和几丁质结合蛋白CBP 21。我们发现转座子插入到chiPQ-的5′非翻译区（5′ UTR）导致几丁质酶缺陷和CBP 21的产生。ChiX小RNA具有与chiPQ-ctbandChiR 5′ UTR互补的序列，可抑制ChiPandChiR的表达。在含有葡萄糖、甘油、GlcNAc和（GlcNAc）2的培养基中检测到ChiX，但仅在含有（GlcNAc）2的培养基中观察到bothchiPandchiR的表达。在含有（GlcNAc）2的培养基中，chiP突变体产生几丁质酶、CBP 21和壳二糖酶，chiP转录物比chiRochiXin的转录物更丰富。这些结果表明，组成型表达的ChiX与高丰度的chiP 5 ′ UTR结合，从而诱导chiRmRNA翻译并随后表达几丁质酶和CBP 21。
Serratia marcescens2170 produces three different types of chitinases and chitin-binding protein CBP21. We found that transposon insertion into the 5′ untranslated region (5′ UTR) ofchiPQ-ctbled to defective chitinase and CBP21 production. ChiX small RNA possessed the complementary sequence of the 5′ UTRs of thechiPQ-ctbandchiRand repressed the expression ofchiPandchiR. ChiX was detected in a medium containing glucose, glycerol, GlcNAc, and (GlcNAc)2, but the expression of bothchiPandchiRwas only observed in a medium containing (GlcNAc)2. ∆chiXmutant produced chitinases, CBP21, and chitobiase without induction.chiPtranscripts were more abundant than those ofchiRorchiXin a medium containing (GlcNAc)2. These results suggest that the constitutively expressed ChiX binds to the highly abundantchiP5′ UTR, thereby leading to the induction ofchiRmRNA translation and the subsequent expression of chitinases and CBP21.