Hierarchical templating in deposition of semi-covalently imprinted inverse opal polythiophene film for femtomolar determination of human serum albumin
Hierarchical templating in deposition of semi-covalently imprinted inverse opal polythiophene film for femtomolar determination of human serum albumin
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DOI：
10.1016/j.bios.2017.02.046
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发表时间：
2017-08-15
影响因子：
12.6
通讯作者：
Kutner, Wlodzimierz
中科院分区：
文献类型：
--
作者：
Dabrowski, Marcin;Cieplak, Maciej;Kutner, Wlodzimierz
设计并制备了具有前所未有的分级结构的纳米人工受体材料，用于测定人血清白蛋白（HSA）。为此，准备了新的分层模板。该模板允许同时控制沉积的分子印迹聚合物（MIP）膜的三个长度尺度上的结构。胶体晶体模板与优化的电化学聚合的2，3 '-联噻吩使得能够沉积反蛋白石形式的MIP膜。沉积的聚合物膜的厚度在印迹的聚（2，3’-联噻吩）膜的恒电位沉积期间用电流振荡的数量精确控制。预先将HSA固定在胶体晶体上允许仅在孔的内表面上形成分子印迹空腔。此外，所有结合位点都位于印迹腔的表面上，其位置对应于HSA分子表面上存在的官能团的位置，这是由于HSA分子与适当的官能单体的预先衍生化。这种协同策略导致材料具有优异的上级识别性能。MIP膜作为识别单元与灵敏的扩展栅场效应晶体管（EG-FET）换能器的集成导致在飞摩尔浓度范围内的高度选择性的HSA测定。
Nanostructured artificial receptor materials with unprecedented hierarchical structure for determination of human serum albumin (HSA) are designed and fabricated. For that purpose a new hierarchical template is prepared. This template allowed for simultaneous structural control of the deposited molecularly imprinted polymer (MIP) film on three length scales. A colloidal crystal templating with optimized electrochemical polymerization of 2,3'-bithiophene enables deposition of an MIP film in the form of an inverse opal. Thickness of the deposited polymer film is precisely controlled with the number of current oscillations during potentiostatic deposition of the imprinted poly(2,3'-bithiophene) film. Prior immobilization of HSA on the colloidal crystal allows formation of molecularly imprinted cavities exclusively on the internal surface of the pores. Furthermore, all binding sites are located on the surface of the imprinted cavities at locations corresponding to positions of functional groups present on the surface of HSA molecules due to prior derivatization of HSA molecules with appropriate functional monomers. This synergistic strategy results in a material with superior recognition performance. Integration of the MIP film as a recognition unit with a sensitive extended-gate field-effect transistor (EG-FET) transducer leads to highly selective HSA determination in the femtomolar concentration range.