SPERM MEMBRANE-POTENTIAL - HYPERPOLARIZATION DURING CAPACITATION REGULATES ZONA PELLUCIDA-DEPENDENT ACROSOMAL SECRETION
SPERM MEMBRANE-POTENTIAL - HYPERPOLARIZATION DURING CAPACITATION REGULATES ZONA PELLUCIDA-DEPENDENT ACROSOMAL SECRETION
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DOI：
10.1006/dbio.1995.1304
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发表时间：
1995-10-01
影响因子：
2.7
通讯作者：
FLORMAN, HM
中科院分区：
文献类型：
--
作者：
ZENG, Y;CLARK, EN;FLORMAN, HM
在小鼠和牛精子群中研究了膜电位（V-M）。V-M由亲脂性阴离子双（1，3-二乙基硫代巴比妥酸）三次甲基oxonol（DiSBAC（2）（3））和亲脂性阳离子3，3 '-二丙基硫代二碳菁碘化物（DiSC（3）（5））的荧光发射测定。对线粒体电位的贡献校正荧光信号，并通过用缬氨霉素或短杆菌肽D选择性透化精子的校准获得表观V-M值。未获能小鼠和牛精子的计算V-M值分别约为-35和-30 mV。相比之下，小鼠和牛精子的获能群体具有-50至-60 mV的V-M值。膜超极化部分是由于K+渗透性增强。获能过程中透明质酸激活的信号转导机制的发展依赖于超极化。这表明，V-M的改变调节精子的激活状态，从而抑制未获能精子的顶体反应，并允许获能精子响应透明质酸刺激。(C)出版社：Academic Press
Membrane potential (V-M) was investigated in mouse and bovine sperm populations. V-M was determined from the fluorescence emission of the lipophilic anion, bis(1,3-diethylthiobarbituric acid)trimethine oxonol (DiSBAC(2)(3)), and from the lipophilic cation, 3,3'-dipropylthiodicarbocyanine iodide (DiSC(3)(5)). Fluorescent signals were corrected for contributions of mitochondrial potentials and apparent V-M values were obtained by calibrations in sperm selectively permeabilized with valinomycin or with gramicidin D. The calculated V-M values of uncapacitated mouse and bovine sperm were approximately -35 and -30 mV, respectively. In contrast, capacitated populations of mouse and bovine sperm have V-M values of -50 to -60 mV. Membrane hyperpolarization is due in part to an enhanced K+ permeability. The development of zona pellucida-activated signal transducing mechanisms during capacitation is dependent upon hyperpolarization. It is suggested that V-M alterations regulate the activation state of sperm, thereby suppressing premature acrosome reactions in uncapacitated sperm and permitting capacitated sperm to respond to zona pellucida stimuli. (C) 1995 Academic Press, Inc.