Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications
Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications
复制标题
用于低自发荧光和生物分析应用的光刻胶 SU-8 的表面改性
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
D. D. Bang
中科院分区：
文献类型：
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作者：
C. Cao;S. Birtwell;Jonas Høgberg;A. Wolff;H. Morgan;D. D. Bang
本文报道了对环氧树脂负性光刻胶SU-8进行表面改性，以降低其自发荧光，同时增强其生物功能性。通过在SU-8表面上共价沉积一薄层20 nm的Au纳米颗粒（AuNPs），我们发现AuNPs涂覆的SU-8表面比未处理的SU-8的荧光少得多。此外，DNA探针可以容易地固定在Au表面上，并且在宽的温度范围内是热稳定的。这些改进将有利于生物分析应用，如DNA杂交和固相PCR（SP-PCR）。
This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).