Elevated levels of interleukin-12 and interferon-gamma in patients with human T lymphotropic virus type I associated myelopathy
Elevated levels of interleukin-12 and interferon-gamma in patients with human T lymphotropic virus type I associated myelopathy
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DOI：
10.1016/s0165-5728(98)00263-x
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发表时间：
1999-03-01
影响因子：
3.3
通讯作者：
Eguchi, K
中科院分区：
文献类型：
--
作者：
Furuya, T;Nakamura, T;Eguchi, K
比较22例人嗜T淋巴病毒I型（HTLV-I）相关脊髓病（HAM）患者血清和脑脊液（CSF）中作为Th1细胞因子的白细胞介素-12 (IL-12) （p70异二聚体）、总IL-12 （p70异二聚体加p40链）、作为Th2细胞因子的干扰素- γ (ifn - γ)，以及作为Th2细胞因子的白细胞介素-4 （IL-4）和白细胞介素-10 （IL-10）的水平，其中9例为抗HTLV-I血清阳性携带者。通过酶联免疫吸附试验（ELISA）测量的血清IL-12（总和p70异源二聚体）和CSF ifn - γ在HAM患者中与OND患者相比显著升高，包括抗htlv - i血清阳性携带者。与对照组相比，HAM患者血清ifn - γ也显著升高。HAM患者脑脊液中IL-12（总和p70异源二聚体）水平与对照组无显著差异，而对于Th2细胞因子IL-4, 13例对照患者中4例抗htlc -1血清阳性携带者脑脊液中检测到IL-4，而HAM患者脑脊液中未检测到IL-4。HAM患者与对照组血清IL-4和IL-10水平无显著差异，CSF IL-10水平也无显著差异。这些结果表明，在HAM患者中，辅助性T淋巴细胞在Th1和Th2之间的免疫平衡倾向于外周的Th1，中枢神经系统中Th1介导的免疫状态参与了HAM的发病机制。(C) 1999 Elsevier Science B.V.版权所有
The levels of interleukin-12 (IL-12) (p70 heterodimer), total IL-12 (p70 heterodimer plus p40 chains), interferon-gamma (IFN-gamma) as Th1 cytokine, and those of interleukin-4 (IL-4) and interleukin-10 (IL-10) as Th2 cytokines in sera and cerebrospinal fluid (CSF) from 22 patients with human T lymphotropic virus type I (HTLV-I)-associated myelopathy (HAM) were compared with those of 22 patients with other neurological diseases (OND), including nine anti-HTLV-I-seropositive carriers. Both serum IL-12 (total and p70 heterodimer) and CSF IFN-gamma, measured by the enzyme-linked immunosorbent assay (ELISA), were significantly elevated in patients with HAM as compared to the patients with OND, including the anti-HTLV-I-seropositive carriers. Serum IFN-gamma also was significantly elevated in the HAM patients as compared to the controls. There was no significant difference in the CSF levels of IL-12 (total and p70 heterodimer) between the HAM patients and controls, whereas, for the Th2 cytokines IL-4 was detected in the CSF of four anti-HTLV-I-seropositive carriers of the 13 control patients but not in any of the patients with HAM. No significant difference was found in the serum levels of IL-4 and IL-10, nor in the CSF levels of IL-10 in the patients with HAM and in the controls. These findings indicate that in patients with HAM, the immunological balance of helper T lymphocytes between Th1 and Th2 is toward Th1 in the periphery and that Th1-mediated immunological status in the central nervous system is involved in the pathogenesis of HAM. (C) 1999 Elsevier Science B.V. All rights reserved.