Functions and functional domains of the GTPase Cdc42p
Functions and functional domains of the GTPase Cdc42p
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DOI：
10.1091/mbc.11.1.339
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发表时间：
2000-01-01
影响因子：
3.3
通讯作者：
Drubin, DG
中科院分区：
文献类型：
--
作者：
Kozminski, KG;Chen, AJ;Drubin, DG
Cdc 42 p是Ras超家族的Rho家族GTdR，是真核生物细胞极性和形态发生的关键调节因子。利用37个cdc 42定点突变体，研究了cdc 42 p在芽殖酵母Saccharomycescerevisiae中的功能及其相互作用。这些cdc 42突变体的细胞学和遗传学分析揭示了新的和不同的表型，表明Cdc 42 p具有至少两个不同的基本功能，并作为一个节点的细胞极性调节在体内。此外，映射每个cdc 42突变的功能数据到一个结构模型的蛋白质揭示作为功能上重要的表面Cdc 42 p是不同于典型的蛋白质相互作用域（开关I，开关II，和C末端）以前确定的成员的Ras超家族。该区域重叠的区域（α 5-螺旋）最近预测的结构模型是一个特异性决定因素Cdc 42 p-蛋白质相互作用。
Cdc42p, a Rho family GTPase of the Ras superfamily, is a key regulator of cell polarity and morphogenesis in eukaryotes. Using 37 site-directed cdc42 mutants, we explored the functions and interactions of Cdc42p in the budding yeast Saccharomyces cerevisiae. Cytological and genetic analyses of these cdc42 mutants revealed novel and diverse phenotypes, showing that Cdc42p possesses at least two distinct essential functions and acts as a nodal point of cell polarity regulation in vivo. In addition, mapping the functional data for each cdc42 mutation onto a structural model of the protein revealed as functionally important a surface of Cdc42p that is distinct from the canonical protein-interacting domains (switch I, switch II, and the C terminus) identified previously in members of the Ras superfamily. This region overlaps with a region (alpha 5-helix) recently predicted by structural models to be a specificity determinant for Cdc42p-protein interactions.