Processing of phosphoenolpyruvate carboxykinase (GTP) RNA in vivo.
Processing of phosphoenolpyruvate carboxykinase (GTP) RNA in vivo.
复制标题
磷酸烯醇丙酮酸羧激酶 (GTP) RNA 的体内加工。
DOI：
10.1073/pnas.82.13.4346
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发表时间：
1985
影响因子：
11.1
通讯作者：
Hanson,RW
中科院分区：
文献类型：
--
作者：
Hatzoglou,M;Sekeris,CE;Hanson,RW
大鼠胞质磷酸烯醇式丙酮酸羧激酶（GTP）（EC 4.1.1.32）mRNA的核RNA前体和尺寸小于成熟酶mRNA的核RNA种类已通过使用对该基因的内含子和外显子具有特异性的杂交探针进行鉴定。注意到许多加工中间体，包括完整的内含子。基于所观察到的前体的大小和分布，我们提出了磷酸烯醇丙酮酸羧激酶RNA加工的途径，该途径涉及从RNA转录物的5'端开始并向3'端进行的内含子剪接。
Nuclear RNA precursors to the mRNA for rat cytosolic phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) and nuclear RNA species smaller in size than the mature enzyme mRNA have been identified by using hybridization probes specific for introns and exons from the gene. A number of processing intermediates, including an intact intron, were noted. Based on the size and distribution of the precursors observed, we propose a pathway for phosphoenolpyruvate carboxykinase RNA processing that involves splicing of introns starting at the 5' end of the RNA transcript and proceeding toward the 3' end.