CULTURE OF PLASMODIUM-FALCIPARUM - THE ROLE OF PH, GLUCOSE, AND LACTATE
CULTURE OF PLASMODIUM-FALCIPARUM - THE ROLE OF PH, GLUCOSE, AND LACTATE
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DOI：
10.2307/3280864
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发表时间：
1983-01-01
影响因子：
1.3
通讯作者：
HELSTOWSKI, LD
中科院分区：
文献类型：
--
作者：
JENSEN, MD;CONLEY, M;HELSTOWSKI, LD
当胞外pH保持在7.2 ~ 7.45之间，胞外乳酸保持在12 mM以下时，恶性疟原虫在体外红细胞培养中的产量最大。宿主红细胞代谢4.6。1.5 .mu。葡萄糖/109红细胞/24小时，产生7.9 +-。1.8 .mu。M乳酸/109红细胞/24小时。34 .mu。M葡萄糖/109寄生红细胞/24小时，产生143 .+-。47 .mu。2 h后，80-100%环型的同步培养比0-25%环型的同步培养在24 h后，每109个被寄生红细胞的糖酵解率明显低于0-25%环型的同步培养。寄生虫生产乳酸所占的葡萄糖利用率明显低于未感染红细胞。这些最佳范围和代谢率可用于寄生虫培养技术的发展。
Yields of P. falciparum in intraerythrocytic in vitro cultures were maximized when extracellular pH was maintained between 7.2 and 7.45, and extracellular lactate was kept below 12 mM. Host erythrocytes metabolized 4.6 .+-. 1.5 .mu.M glucose/109 RBC[red blood cell]/24 h and produced 7.9 .+-. 1.8 .mu.M lactate/109 RBC/24 h. Asynchronous parasite cultures used 122 .+-. 34 .mu.M glucose/109 parasitized RBC/24 h and produced 143 .+-. 47 .mu.M lactate/109 parasitized RBC/24 h. Synchronous cultures that were 80-100% ring forms after 2 h in culture exhibited significantly lower glycolysis per 109 parasitized RBC than cultures that were 0-25% ring forms after 24 h. The percent of glucose utilization accounted for by lactate production by parasites was significantly less than that of uninfected erythrocytes. These optimum ranges and metabolic rates can be used in the development of parasite culture techniques.