Modulatory effects of perineuronal oligodendrocytes on neuronal activity in the rat hippocampus
Modulatory effects of perineuronal oligodendrocytes on neuronal activity in the rat hippocampus
复制标题
神经周围少突胶质细胞对大鼠海马神经元活动的调节作用
DOI：
10.1007/s11064-017-2278-9
复制
发表时间：
2018
影响因子：
4.4
通讯作者：
Fujii S
中科院分区：
文献类型：
--
作者：
Yamazaki Y;Hozumi Y;Kaneko K;Fujii S
动作电位是神经系统中从一个区域传递到另一个区域的信息的基础。神经回路中动作电位放电模式的变化会影响大脑处理信息的方式。在我们之前的研究中，我们关注海马 CA1 区的中间神经元/神经周围星形胶质细胞对，并报道神经元周围星形胶质细胞的直接去极化调节中间神经元的放电模式。在当前的研究中，我们研究了神经周围少突胶质细胞的形态和电生理特性，并检查了它们对 CA1 区神经元间放电的调节作用。神经元周围少突胶质细胞只有少数突起，这些突起弯曲、复杂地扭曲，缠绕在邻近中间神经元主突起的胞体和近端区域。神经周围少突胶质细胞的全细胞电流模式是均匀的，电流-电压关系显示出显着的外向整流。尽管 K+ 通道阻滞剂四乙铵和 4-氨基吡啶明显阻断了外向电流，但 Ba2+ 并未显着改变全细胞电流。与神经周围星形胶质细胞不同，神经周围少突胶质细胞的去极化对神经元间放电没有影响。然而，当中间神经元以更高的频率放电时，神经元周围少突胶质细胞的超极化抑制了它们的动作电位。神经周围少突胶质细胞的抑制作用在低浓度四乙铵存在下受到抑制，选择性地阻止深度和快速的后超极化。这些结果表明，神经元周围少突胶质细胞通过影响 K+ 通道来抑制神经元间放电，而 K+ 通道负责深度和快速的后超极化。
Action potentials are fundamental to relaying information from region to region in the nervous system. Changes in action potential firing patterns in neural circuits influence how the brain processes information. In our previous study, we focused on interneuron/perineuronal astrocyte pairs in the hippocampal CA1 region and reported that direct depolarization of perineuronal astrocytes modulated the firing pattern of interneurons. In the current study, we investigated the morphological and electrophysiological properties of perineuronal oligodendrocytes, and examined their modulatory effects on interneuronal firing in the CA1 region. Perineuronal oligodendrocytes only had a few processes, which were crooked, intricately twisted, and twined around the soma and proximal region of the main processes of adjacent interneurons. Whole-cell current patterns of perineuronal oligodendrocytes were homogenous and the current–voltage relationship showed remarkable outward rectification. Although the K+channel blockers, tetraethylammonium and 4-aminopyridine, clearly blocked outward currents, Ba2+did not significantly alter whole-cell currents. Unlike perineuronal astrocytes, the depolarization of perineuronal oligodendrocytes had no effect on interneuronal firing; however, when the interneurons were firing at a higher frequency, the hyperpolarization of perineuronal oligodendrocytes suppressed their action potentials. The suppressive effects of perineuronal oligodendrocytes were inhibited in the presence of a low concentration of tetraethylammonium, which selectively blocked deep and fast afterhyperpolarization. These results suggest that perineuronal oligodendrocytes suppress interneuronal firing through their influence on K+channels, which are responsible for deep and fast afterhyperpolarization.