Protein aggregation studied by forward light scattering and light transmission analysis
Protein aggregation studied by forward light scattering and light transmission analysis
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DOI：
10.1016/j.chemphys.2007.09.014
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发表时间：
2007-12-06
期刊：
影响因子：
2.3
通讯作者：
Wolf, E.
中科院分区：
文献类型：
--
作者：
Penzkofer, A.;Shirdel, J.;Wolf, E.
通过蛋白质透明波长区域的透射和前向光散射测量，研究了果蝇 (dCry) 昼夜节律蓝光光感受器隐花色素的聚集。向前方向的光散射是由瑞利散射引起的，其与聚集程度成正比。通过透明区域中的样品的光透射因各个方向上的 Mic 光散射而减少。它取决于聚集体的聚集程度和单体体积填充因子（随着蛋白质球单体体积填充因子的降低，总散射减少），从而可以区分紧密堆积的蛋白质聚集（单体体积填充因子 1）和松散堆积的蛋白质聚集（单体体积填充因子小于 1）。观察到聚集随着温度、浓度和蓝光暴露的增加而增加。在 4 摄氏度和低于 0.135 mM 的蛋白质浓度下，未观察到 dCry 聚集，而在 24 摄氏度和 0.327 mM 下，发生凝胶化（松散堆积的聚集体占据整个溶液体积）。 (C) 2007 Elsevier B.V. 保留所有权利。
The aggregation of the circadian blue-light photo-receptor cryptochrome from Drosophila melanogaster (dCry) is studied by transmission and forward light scattering measurement in the protein transparent wavelength region. The light scattering in forward direction is caused by Rayleigh scattering which is proportional to the degree of aggregation. The light transmission through the samples in the transparent region is reduced by Mic light scattering in all directions. It depends on the degree of aggregation and the monomer volume fill factor of the aggregates (less total scattering with decreasing monomer volume fill factor of protein globule) allowing a distinction between tightly packed protein aggregation (monomer volume fill factor 1) and loosely packed protein aggregation (monomer volume fill factor less than 1). An increase in aggregation with temperature, concentration, and blue-light exposure is observed. At a temperature of 4 degrees C and a protein concentration of less than 0.135 mM no dCry aggregation was observed, while at 24 degrees C and 0.327 mM gelation occurred (loosely packed aggregates occupying the whole solution volume). (C) 2007 Elsevier B.V. All rights reserved.