Kinetics of cellular proliferation after arterial injury. V. Role of acute distension in the induction of smooth muscle proliferation.
Kinetics of cellular proliferation after arterial injury. V. Role of acute distension in the induction of smooth muscle proliferation.
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发表时间：
1989-03
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通讯作者：
A. Clowes;Clowes Mm;J. Fingerle;M. Reidy
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作者：
A. Clowes;Clowes Mm;J. Fingerle;M. Reidy
通过球囊导管剥脱的动脉表现出明显的平滑肌细胞（SMC）增殖，而通过细钢丝剥脱的动脉则没有。我们检查了球囊模型中血管扩张以及内皮损失导致SMC增殖的可能性。通过暂时结扎分离的大鼠左颈动脉段通过颈外动脉插管在300 mm Hg压力下用组织培养基流体静力学扩张。动物接受连续[3 H]胸苷输注（腹膜内，Alzet泵，Alz Corp.，Palo Alza，加州）14天或在第1、2、3或7天用[3 H]胸苷脉冲，并通过放射自显影术评估SMC增殖。扩张的血管表现出斑片状剥脱急性和完整的内皮再生3天。随后内膜中不存在SMC。然而，14天内累积SMC增殖为21 +/- 3%（灌注但未扩张对照组：0.8 +/- 0.6%;部分去内皮化但未扩张对照组：0.3 +/- 0.1%）。脉冲标记显示，SMC增殖的高峰在损伤后2 - 3天之间。这些结果表明，急性扩张可以促进内皮剥脱后SMC增殖的诱导。
Arteries denuded by the passage of a balloon catheter demonstrate marked smooth muscle cell (SMC) proliferation, whereas arteries denuded by a fine wire do not. We examined the possibility that vessel distension in addition to endothelial loss accounted for the SMC proliferation in the balloon model. Segments of rat left carotid isolated by temporary ligatures were distended hydrostatically with tissue culture medium at 300 mm Hg pressure via an external carotid cannula. Animals received either continuous [3H]thymidine infusion (intraperitoneally, Alzet pump, Alz Corp., Palo Alza, California) for 14 days or pulsed with [3H]thymidine at 1, 2, 3, or 7 days, and SMC proliferation was assessed by autoradiography. Distended vessels demonstrated patchy denudation acutely and complete endothelial regeneration by 3 days. No SMC were present in the intima at later times. Nevertheless, cumulative SMC proliferation over 14 days was 21 +/- 3% (perfused but not distended control: 0.8 +/- 0.6%; partially de-endothelialized but not distended control: 0.3 +/- 0.1%). Pulse-labeling demonstrated a peak of SMC proliferation between 2 and 3 days after injury. These results suggest that acute distension can contribute to the induction of SMC proliferation after endothelial denudation.