Surfactant protein A inhibits the non-enzymatic lipid peroxidation of porcine lung surfactant
Surfactant protein A inhibits the non-enzymatic lipid peroxidation of porcine lung surfactant
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DOI：
10.1054/plef.2001.0309
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发表时间：
2001-10-01
影响因子：
3
通讯作者：
Catalá, A
中科院分区：
文献类型：
--
作者：
Blanco, O;Catalá, A
在37 ℃孵育180 min期间，通过测量光发射-化学发光来评估表面活性剂蛋白A（SP-A）抑制抗坏血酸-Fe 2+诱导的猪肺表面活性剂（surfacen）中发现的多不饱和脂肪酸的脂质过氧化的能力。发光（化学发光）是浓度依赖性的。当肺表面活性物质在抗坏血酸-Fe ~（2+）体系中孵育时，观察到表面活性物质脂肪酸组成的变化。主要的多不饱和脂肪酸C18：2 n6和C20：4 n6肺表面活性物质中发现180分钟的脂质过氧化过程后大大减少。从猪肺分离的天然SP-A以剂量依赖性方式抑制表面活性剂长链多不饱和脂肪酸（主要是花生四烯酸）的氧化，其在2.0 μ g/ml浓度下为半最大值（60%抑制），在4.0 μ g/ml浓度下几乎完全（73.6%抑制），如通过抑制光发射和脂肪酸组成分析所示。在肺SP-A的最高浓度下，观察到大多数多不饱和脂肪酸的保护与光发射的抑制之间存在非常好的相关性。(C)2001年哈考特出版社有限公司
The ability of surfactant protein A (SP-A) to inhibit the ascorbate-Fe2+ induced lipid peroxidation of polyunsaturated fatty acids found in porcine lung surfactant (surfacen) was assessed by measuring the light emission - chemiluminescence during a 180-min incubation period at 37 degreesC. The light emission (chemiluminescence) was concentration dependent. Changes in the fatty acid composition of surfacen were observed when the lung surfactant was incubated in an ascorbate-Fe2+ system. The main polyunsaturated fatty acids C18:2 n6 and C20:4 n6 found in the lung surfactant decreased considerably after a 180-min lipid peroxidation process. Native SP-A isolated from pig lungs inhibited oxidation of surfactant long chain polyunsaturated fatty acids, mainly arachidonic acid, in a dose-dependent fashion that was half-maximal (60% inhibition) at a concentration of 2.0 mug/ml and almost complete (73.6% inhibition) at 4.0 mug/ml, as indicated by inhibition of light emission and fatty acid composition analysis. At the highest concentration of lung SP-A used a very good correlation between the protection of the most polyunsaturated fatty acids and inhibition of light emission was observed. (C) 2001 Harcourt Publishers Ltd.