RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans
RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans
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DOI：
10.1016/s0960-9822(00)00869-1
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发表时间：
2000-12-14
期刊：
影响因子：
9.2
通讯作者：
Kemphues, KJ
中科院分区：
文献类型：
--
作者：
Piano, F;Schetter, AJ;Kemphues, KJ
作为对基因组进行全面功能分析的一步，系统的基因敲除项目已经在几种生物体中启动[1]。在C.然而，在线虫中，母体的贡献可以掩盖基因敲除对胚胎发生的影响。RNA干扰（RNAi）提供了一种替代的快速方法来获得功能丧失的信息，也可以揭示靶向基因的胚胎作用[2，3]。我们使用RNAi分析了一组随机的卵巢转录本，并鉴定了81个在胚胎发生中起重要作用的基因，令人惊讶的是，它们都没有定位在X染色体上。在这81个基因中，有68个在八细胞期之前表现出缺陷，可以分为10个表型类。为了存档和分发这些数据，我们开发了一个直接与C。elegans数据库（Wormbase）。我们的结论是，筛选cDNA文库的RNAi是一种有效的方法，获得在体内功能的一大群基因。此外，这种方法可直接应用于对RNAi敏感且其基因组尚未测序的其他生物。
As a step towards comprehensive functional analysis of genomes, systematic gene knockout projects have been initiated in several organisms [1]. In metazoans like C. elegans, however, maternal contribution can mask the effects of gene knockouts on embryogenesis. RNA interference (RNAi) provides an alternative rapid approach to obtain loss-of-function information that can also reveal embryonic roles for the genes targeted [2,3]. We have used RNAi to analyze a random set of ovarian transcripts and have identified 81 genes with essential roles in embryogenesis, Surprisingly, none of them maps on the X chromosome. Of these 81 genes, 68 showed defects before the eight-cell stage and could be grouped into ten phenotypic classes. To archive and distribute these data we have developed a database system directly linked to the C. elegans database (Wormbase). We conclude that screening cDNA libraries by RNAi is an efficient way of obtaining in vivo function for a large group of genes. Furthermore, this approach is directly applicable to other organisms sensitive to RNAi and whose genomes have not yet been sequenced.