Diversity of Microcystis aeruginosa isolates (Chroococcales, Cyanobacteria) from East-African water bodies
Diversity of Microcystis aeruginosa isolates (Chroococcales, Cyanobacteria) from East-African water bodies
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DOI：
10.1007/s00203-007-0219-8
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发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Edvardsen, Bente
中科院分区：
文献类型：
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作者：
Haande, Sigrid;Ballot, Andreas;Edvardsen, Bente
除南非外，人们对非洲大陆上有毒蓝藻和蓝藻水华的存在和丰富知之甚少。由于社会与自然关系密切，非洲大部分地区都将稀少的水资源用作饮用水，因此人们认识到，更多地了解有毒蓝藻水华是非常重要的。已知形成铜绿微囊藻的水华会产生对人类有毒的环状七毒毒素(微囊藻毒素)。本研究对来自肯尼亚和乌干达几个水体的24株铜绿假单胞菌的形态、遗传和化学特性进行了研究，其中一些作为饮用水源。不同的鉴定方法，铜绿假单胞菌具有不同程度的多样性。根据传统的形态学方法鉴定出4种形态类型，通过PC-IGS和ITS1 rDNA区域的DNA序列比较鉴定出10种基因型，基于MALDI-TOF-MS寡肽分析鉴定出10种化学类型。在来自东非的24个分离菌株中，只有4个菌株产生微囊藻毒素，而大多数菌株都检测到属于铜绿假单胞菌素和氰神经节苷类的寡肽。
With exception of South Africa, very little is known about the presence and abundance of toxic cyanobacteria and cyanobacterial blooms on the African continent. The close proximity between society and nature, and the use of the sparse water resources as drinking water in large parts of Africa, lead to the recognition that more knowledge on toxic cyanobacterial blooms is of major importance. The bloom forming cyanobacterium Microcystis aeruginosa is known to produce cyclic heptatoxins (microcystins) which can be toxic to humans. In this study the morphological, genetic, and chemical characters of 24 strains of M. aeruginosa from several water bodies in Kenya and Uganda, some of them used as drinking water sources, were examined. The M. aeruginosa strains possessed different levels of diversity depending on characterisation method. Four morphotypes were identified based on the traditional morphological approach, 10 genotypes by DNA sequence comparison of the PC-IGS and ITS1 rDNA regions, and 10 chemotypes based on MALDI-TOF-MS oligopeptide analysis. Only 4 of the 24 isolated strains from East Africa were found to produce microcystins, while oligopeptides belonging to the aeruginosin and cyanopeptolin class were detected in most strains.