DOCOSAHEXAENOIC ACID (22-6, N-3) IS METABOLIZED TO LIPOXYGENASE REACTION-PRODUCTS IN THE RETINA
DOCOSAHEXAENOIC ACID (22-6, N-3) IS METABOLIZED TO LIPOXYGENASE REACTION-PRODUCTS IN THE RETINA
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DOI：
10.1016/0006-291x(84)90601-6
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
REDDY, TS
中科院分区：
文献类型：
--
作者：
BAZAN, NG;BIRKLE, DL;REDDY, TS
二十二碳六烯酸是视网膜磷脂的主要成分，是与[U-14C]二十二碳六烯酸体外孵育的完整犬视网膜中活性脂氧合的底物。通过高效液相色谱和气相色谱-质谱法鉴定主要脂氧合酶反应产物为11-羟基-4,7,9-(反式)13,16,19二十二碳六烯酸。还检测到其他单羟基和二羟基衍生物。这些化合物的合成受到抗氧化剂和脂氧合酶抑制剂去甲二氢愈创木酸的抑制，但不被吲哚美辛或七叶亭抑制。
Docosahexaenoic acid, a major component of retinal phospholipids, is a substrate for active lipoxygenation in intact canine retinas incubated in vitro with [U-14C]docosahexaenoic acid. The major lipoxygenase reaction product was identified by high performance liquid chromatography and gas chromatography-mass spectrometry as 11-hydroxy-4,7,9-(trans)13,16,19 docosahexaenoic acid. Other mono- and di-hydroxy derivatives were also detected. The synthesis of these compounds was inhibited by the antioxidant and lipoxygenase inhibitor, nordihydroguaiaretic acid, but was not inhibited by indomethacin or esculetin.