Epithelial and Connective Tissue Sealing around Titanium Implants with Various Typical Surface Finishes
Epithelial and Connective Tissue Sealing around Titanium Implants with Various Typical Surface Finishes
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DOI：
10.1021/acsbiomaterials.9b00499
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发表时间：
2019-10-01
影响因子：
5.8
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Narimatsu, Ikue;Atsuta, Ikiru;Koyano, Kiyoshi
牙种植体周围的软组织屏障在牙种植体的成功中起着至关重要的作用，因为它保护了下面的硬组织结构。近年来，许多种植体的表面改性技术被用来改善骨-种植体的接触，但对种植体周围软组织（PIS）的研究却很少。本研究的重点是各种典型表面光洁度的植入物周围上皮和结缔组织密封的“生物宽度”，通过测试已加工（Ms）、喷砂和酸蚀刻粗糙化（Rs）、CaCl 2水热处理（Cs）或阳极氧化（As）的表面。MS、Rs和As技术通常用于对市售牙植入物的表面进行抛光。据报道，Cs技术可产生强的上皮细胞-钛粘附。在培养研究中，将大鼠口腔上皮细胞（OECs）和成纤维细胞培养在Ms、Rs、Cs和As钛板上。在Rs和As培养板上培养的嗅鞘细胞贴壁率低于Ms和Cs培养板，胶原蛋白表达量高于Ms和Cs培养板。对于体内研究，将具有Ms、Rs、Cs和As表面的植入物置于大鼠口腔中。虽然PIS结构与天然牙周围的结构相似，但辣根过氧化物酶测定显示，Ms和Rs种植体周围的密封能力弱于Cs种植体周围的密封能力。16周后，Rs种植体表现出种植体周围上皮顶端向下生长，并失去了骨支持。因此，尽管光滑表面（Ms和Cs）显示出更好的上皮附着，但粗糙表面（Rs和As）更适合于结合至结缔组织。牢固的上皮-种植体附着似乎是防止异物穿透的基本防御。选择合适的表面以确保牢固的密封对于种植体的成功非常重要。
Soft tissue barrier around a dental implant plays a crucial role in the success of dental implants because it protects underlying hard tissue structures. A number of surface alteration procedures of implants have been introduced to improve bone-implant contact, but there has been little research on the peri-implant soft tissue (PIS) seal. The present study focuses on the "biologic width" of epithelial and connective tissue seals around implants with various typical surface finishes by testing surfaces that have been machined (Ms), roughened by sandblasting and acid etching (Rs), treated hydrothermally with CaCl2 (Cs), or anodized (As). Ms, Rs, and As techniques are commonly used to finish surfaces of commercially available dental implants. The Cs technique was reported to produce strong epithelial cell-titanium adhesion. For culture study, rat oral epithelial cells (OECs) and fibroblasts were cultured on Ms, Rs, Cs, and As titanium plates. There was less cell adherence of OECs and more collagen expression when cultured on Rs and As plates than when cultured on Ms and Cs plates. For the in vivo study, implants with Ms, Rs, Cs, and As surfaces were placed in the rats' oral cavity. Although the PIS structure was similar to that around natural teeth, a horseradish peroxide assay revealed that the sealing ability around the Ms and Rs implants was weaker than that around Cs implants. After 16 weeks, Rs implants exhibited peri-implant epithelial apical down-growth and had lost bone support. Thus, although a smooth surface (Ms and Cs) showed better epithelial attachment, rough surfaces (Rs and As) are more suitable for binding to the connective tissue. Strong epithelium-implant attachment seems to be a fundamental defense against foreign body penetration. Selecting suitable surfaces to ensure strong sealing is important for implant success.