Induction of murine TNBS colitis is strictly controlled by a modified method using continuous inhalation anesthesia with sevoflurane.
Induction of murine TNBS colitis is strictly controlled by a modified method using continuous inhalation anesthesia with sevoflurane.
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通过使用七氟烷连续吸入麻醉的改良方法严格控制小鼠 TNBS 结肠炎的诱导。
DOI：
10.1007/s10620-013-3023-0
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发表时间：
2014
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et al.
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作者：
Tomohiro Terai;Ken Sugimoto;et al.
背景三硝基苯磺酸（TNBS）诱导的结肠炎是应用最广泛的结肠炎模型之一。然而，由于难以实现结肠炎的均匀分布，尚无TNBS诱导结肠炎的标准程序。我们已经开发了一种改进的小鼠TNBS诱导结肠炎的方法，包括七氟醚吸入麻醉联合单次和多次TNBS给药。目的比较新方法与常规腹腔麻醉诱导小鼠tnbs性结肠炎的有效性。方法采用七氟醚连续吸入麻醉和2.5%阿维汀常规腹腔注射两种方法，分别对C57BL/6J小鼠倒立体位给予乙醇中的stnbs。在结肠炎过程中检查体重变化、细胞因子谱和组织学结果。结果与常规方法相比，新方法在结肠炎过程中麻醉弥散时间、TNBS滞留时间和最低点重量均有所减少。经改良的TNBS诱导的结肠炎在第7天得到优化，Th1和Th17细胞因子在第7天的显著表达，在55%乙醇中注射2.25 mg TNBS。七氟醚吸入麻醉调节TNBS滞留时间，可严格控制TNBS诱导结肠炎的病情严重程度。使用改进的方法，我们也能够通过反复给药TNBS建立慢性TNBS诱导的结肠炎模型，而没有过量的小鼠死亡。结论七氟醚连续吸入麻醉小鼠TNBS诱导结肠炎的sour修饰方法提供了单次和多次给药TNBS后较好的实验性结肠炎模型。
BackgroundTrinitrobenzenesulfonic acid (TNBS)-induced colitis is one of the most widely used experimental colitis models. However, there is no standard procedure for inducing colitis by TNBS because it is difficult to achieve a uniform distribution of colitis. We have developed a modified method of murine TNBS-induced colitis that involves inhalation anesthesia with sevoflurane combined with both single and repeated TNBS administrations.AimsTo compare the usefulness of our newly developed method for inducing murine TNBS-induced colitis with that of conventional intraperitoneal anesthesia.MethodsTNBS in ethanol was administered to C57BL/6J mice held in an inverted vertical position either under continuous inhalation anesthesia with sevoflurane, in accordance with our newly developed method, or by intraperitoneal injection with 2.5 % avertin, in accordance with the conventional procedure. Body weight change, cytokine profile, and histological findings were examined during the course of colitis.ResultsThe dispersion of anesthesia time, TNBS retention time, and nadir weight during the course of colitis was decreased using the newly developed method compared with the conventional procedure. Optimization of the modified TNBS-induced colitis, as evidenced by the predominant expression of Th1 and Th17 cytokines on day 7, was attained by the injection of 2.25 mg TNBS in 55 % ethanol. Regulation of the TNBS retention time using inhalation anesthesia with sevoflurane allowed strict control of the disease severity of TNBS-induced colitis. Using the modified method we were also able to develop a chronic TNBS-induced colitis model by repeated TNBS administration without excessive mortality of the mice.ConclusionsOur modified method for murine TNBS-induced colitis using continuous inhalation anesthesia with sevoflurane provides a better experimental colitis model following both single and repeated TNBS administrations.