Are intratumoral microbiota involved in the progression of intraductal papillary mucinous neoplasms of the pancreas?
Are intratumoral microbiota involved in the progression of intraductal papillary mucinous neoplasms of the pancreas?
复制标题
DOI：
10.1016/j.surg.2022.10.003
复制
发表时间：
2023-01-28
期刊：
影响因子：
3.8
通讯作者：
Ohtsuka, Takao
中科院分区：
文献类型：
--
作者：
Hozaka, Yuto;Oi, Hideyuki;Ohtsuka, Takao
背景资料：据报道，微生物群通过持续炎症机制影响各种胃肠道肿瘤的发展;然而，关于其对导管内乳头状粘液性肿瘤的影响的数据很少。本研究的目的是评估特定微生物群与胰腺导管内乳头状粘液性肿瘤临床病理特征之间的关系。从30例因导管内乳头状粘液性肿瘤接受胰腺切除术的患者的福尔马林固定、石蜡包埋样本中提取DNA，使用16 S重组DNA的V3和V4区的引物组，使用聚合酶链反应来创建序列文库。然后将过滤的序列读数处理成具有97%同一性阈值的操作分类单位，并使用操作分类单位比较两组之间的细菌相对丰度。结果如下：主导管操作分类单元中厚壁菌门的相对丰度比分支导管操作分类单元中厚壁菌门的相对丰度低，变形菌门和梭杆菌门的相对丰度高。侵袭性导管内乳头状黏液性肿瘤中拟杆菌（P <0.01）和梭杆菌（P = 0.04）的相对丰度显著高于非侵袭性导管内乳头状黏液性肿瘤。厚壁菌门中肠型的相对丰度显著低于非肠型的相对丰度（P = 0.04）。值得注意的是，主管道操作分类单位与肠亚型的变形菌门和梭菌门的比例增加的影响，和梭菌门是丰富的肠型侵入性主管道操作分类units.Conclusion：Intratomoral微生物群可能参与的进展的操作分类单位。(c)2022爱思唯尔公司All rights reserved.
Background: Microbiota have been reported to influence the development of various gastrointestinal neoplasms through the mechanism of sustained inflammation; however, few data are available regarding their influence on intraductal papillary mucinous neoplasms. The aim of this study was to assess the association between specific microbiota and the clinicopathologic characteristics of intraductal papillary mucinous neoplasms of the pancreas.Methods: DNA was extracted from formalin-fixed, paraffin-embedded samples of 30 patients who underwent pancreatectomy for intraductal papillary mucinous neoplasm, and polymerase chain reaction was used to create sequence libraries using the primer set for the V3 and V4 region of 16S recombinant DNA. Filtered sequence reads were then processed into operational taxonomic units with a 97% identity threshold and the relative abundance of bacteria compared between the 2 groups using operational taxonomic units. Results: There was a trend toward fewer Firmicutes and more Proteobacteria and Fusobacteria in the relative abundance of main duct operational taxonomic units than in branch duct operational taxonomic units. The relative abundances of Bacteroidetes (P < .01) and Fusobacteria (P = .04) were significantly higher in invasive intraductal papillary mucinous neoplasms than in noninvasive intraductal papillary mucinous neoplasms. The relative abundance of the intestinal type was significantly lower in Firmicutes than the relative abundance of the nonintestinal type (P = .04). Notably, main duct operational taxonomic units with the intestinal subtype were affected by increased proportions of Proteobacteria and Fusobacteria, and Fusobacteria were abundant in the intestinal type of invasive main duct operational taxonomic units.Conclusion: Intratumoral microbiota may be involved in the progression of operational taxonomic units.(c) 2022 Elsevier Inc. All rights reserved.