Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue
Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue
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DOI：
10.1563/aaid-joi-d-12-00027
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发表时间：
2013-08-01
影响因子：
1.6
通讯作者：
Jahangir, Shahrbanoo
中科院分区：
文献类型：
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作者：
Khorsand, Afshin;Eslaminejad, Mohamadreza Baghaban;Jahangir, Shahrbanoo
本研究旨在探讨牙髓干细胞（DPSCs）对实验性犬牙周组织缺损再生的影响。本文对10只杂种狗的第一个下前磨牙进行了手术制造的近中三壁牙周缺损合并结扎性牙周炎。同时，从同一犬的上颌前磨牙中提取DPSCs。牙周炎模型建立4周后，将自体传代-3型DPSCs联合Bio-Oss植入一侧作为实验组。在另一边，只有Bio-Oss被植入作为对照。术后8周，从骨组织、牙周韧带（PDL）和骨水泥形成的角度对牙周缺损的再生进行组织学和组织形态计量学评估。组织学上，在所有的试验标本（10个缺陷）中，观察到骨质、骨和PDL的再生。在对照组中，虽然我们观察到所有缺损的骨再生，但在9个缺损中发现骨骨质形成，8个缺损中发现PDL。组织光度分析显示，试验组再生牙骨质数量和PDL（分别为3.83 +/- 1.32 mm和3.30 +/- 1.12 mm）显著高于对照组（分别为2.42 +/- 1.40 mm和1.77 +/- 1.27 mm）；由DPSCs和bio - os组成的生物复合物在牙周组织再生中具有广阔的应用前景。
This study aimed to investigate effects of dental pulp stem cells (DPSCs) on regeneration of a defect experimentally created in the periodontium of a canine model. Surgically created mesial 3-walled periodontal defects with ligature-induced periodontitis were produced bilaterally in the first lower premolar teeth of 10 mongrel dogs. Simultaneously, DPSCs were derived from the maxillary premolar teeth of the same dogs. Four weeks after creation of the periodontitis model, autologous passaged-3 DPSCs combined with Bio-Oss were implanted on one side as the test group. On the other side, only Bio-Oss was implanted as a control. Eight weeks after surgery, regeneration of the periodontal defects was evaluated histologically and histomorphometrically in terms of bone, periodontal ligament (PDL), and cement formation. Histologically, in all test specimens (10 defects), regeneration of cementum, bone, and PDL was observed. In the control groups, although we observed the regeneration of bone in all defects, the formation of cementum was seen in 9 defects and PDL was seen in 8 defects. Histonnorphometric analyses showed that the amount of regenerated cementum and PDL in the test groups (3.83 +/- 1.32 mm and 3.30 +/- 1.12 mm, respectively) was significantly higher than that of the control groups (2.42 +/- 1.40 mm and 1.77 +/- 1.27 mm, respectively; P < .05). A biocomplex consisting of DPSCs and Bio-Oss would be promising in regeneration of periodontal tissues.