Molecular characterization of Salmonella spp. isolated from bulk tank milk and cull dairy cow fecal samples
Molecular characterization of Salmonella spp. isolated from bulk tank milk and cull dairy cow fecal samples
复制标题
DOI：
10.4315/0362-028x-65.7.1100
复制
发表时间：
2002-07-01
影响因子：
2
通讯作者：
Oliver, SP
中科院分区：
文献类型：
--
作者：
Murinda, SE;Nguyen, LT;Oliver, SP
食用淘汰奶牛的肉和生牛奶与食源性沙门氏菌病有关。本次调查的目的是建立沙门氏菌的患病率剔除奶牛粪便样本和散装罐牛奶，并确定沙门氏菌阳性奶牛场（n = 30）在田纳西州东部的比例。食品和药物管理局细菌学分析方案通常用于沙门氏菌分离。用乳糖肉汤进行初级富集。用连四硫酸盐肉汤进行二次富集。曙红亚甲蓝。Hektoen肠，木糖赖氨酸脱氧胆酸盐。亚硫酸铋和亮绿色（BG）作为分离琼脂。还评价了补充有单独抗生素和/或硫化合物的BG琼脂。从30个奶牛场中的7个（25.3%）的268份散装罐牛奶样品中的6份（2.24%）和415份粪便样品中的9份（2.17%）为沙门氏菌阳性。大多数分离株（15）9月和12月之间获得。沙门氏菌分离株进一步表征使用多价体0沙门氏菌抗血清，邻硝基苯基-β-D-吡喃半乳糖苷（ONPG）。和肠杆菌科的分析特征指数（API）20 E条。假定阳性沙门氏菌分离株的血清学评价导致大量的假阳性（41.2%）。ONPG和API 20 E检测能够进一步区分大多数沙门氏菌属，与亚利桑那沙门氏菌和肠杆菌的密切相关成员（如柠檬酸杆菌）。SpeI消化沙门氏菌DNA的脉冲场凝胶电泳用于分型分离株。分离物分为四个簇。本次调查产生的基线信息正被用于在选定的农场制定收获前病原体减少计划。
The consumption of meat from cull dairy cows and of raw milk has been associated with foodborne salmonellosis. This survey was conducted to establish the prevalence of Salmonella in cull dairy cow fecal samples and bulk tank milk and to determine the proportion of Salmonella-positive dairy farms (n = 30) in east Tennessee. Food and Drug Administration bacteriological analytical protocols were generally used for Salmonella isolation. Primary enrichment was performed with lactose broth. and secondary enrichment was conducted with tetrathionate broth. Eosin methylene blue. hektoen enteric, xylose lysine desoxycholate. bismuth sulfite. and brilliant green (BG) were used as isolation agars. BG agars supplemented with individual antibiotics and/or Sulfur compounds were also evaluated. Six of 268 (2.24%) bulk tank milk samples and 9 of 415 (2.17%) fecal samples from 7 of 30 (25.3%) dairy farms were Salmonella-positive. Most isolates (I I of 15) were obtained between September and December. Salmonella isolates were further characterized using polyvalent somatic 0 Salmonella antiserum, o-nitrophenyl-beta-D-galactopyranoside (ONPG). and Analytical Profile Index (API) 20E strips for Enterobacteriaceae. Serological evaluation of presumptive positive Salmonella isolates resulted in substantial numbers of false positives (41.2%). ONPG and API 20E tests enabled further biochemical distinction of the majority of Salmonella spp, from Salmonella Arizonae and closely related members of Enterobacteriaceue like Citrobacter youngae. Pulsed-field gel electrophoresis of SpeI-digested Salmonella DNA was used to subtype isolates. The isolates grouped into four clusters. The baseline information generated in this survey is being used to develop preharvest pathogen reduction programs on selected farms.