In vivo magnetic resonance imaging tracks adult neural progenitor cell targeting of brain tumor
In vivo magnetic resonance imaging tracks adult neural progenitor cell targeting of brain tumor
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DOI：
10.1016/j.neuroimage.2004.05.019
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发表时间：
2004-09-01
期刊：
影响因子：
5.7
通讯作者：
Chopp, M
中科院分区：
文献类型：
--
作者：
Zhang, ZG;Jiang, Q;Chopp, M
我们使用磁共振成像（MRI）描述了一种无创追踪大鼠脑肿瘤中移植的神经祖细胞和骨髓基质细胞（MSC）的方法。神经祖细胞和间充质干细胞用亲脂性染料涂层的超顺磁颗粒进行标记。 9L-胶质瘤细胞植入1周后，将标记的神经祖细胞和MSC分别通过小脑延髓池和尾静脉移植到大鼠体内。三维 (3D) 梯度回波和造影剂图像显示，通过 MRI 信号丢失检测到成体神经祖细胞和 MSC 向肿瘤块和浸润肿瘤细胞的动态迁移。普鲁士蓝染色和荧光显微镜分析表明，移植细胞靶向肿瘤细胞，并且移植细胞的区域对应于MRI信号丢失的区域。这些结果表明，MRI 技术为体内评估靶向肿瘤块和浸润肿瘤细胞的移植细胞提供了一种灵敏的方法，并且成体神经祖细胞和 MSC 可以靶向大脑中的肿瘤聚集体。 (C) 2004 Elsevier Inc. 保留所有权利。
Using magnetic resonance imaging (MRI), we described a method for noninvasively tracking grafted neural progenitor cells and bone marrow stromal cells (MSCs) in brain tumor of the rat. Neural progenitor cells and MSCs were labeled with lipophilic dye-coated superparamagnetic particles. The labeled neural progenitor cells and MSCs were transplanted to rats via the cisterna magna and a tail vein, respectively, 1 week after 9L-gliosarcoma cell implantation. Three-dimensional (3D) gradient echo and contrast agent images revealed dynamic migration of adult neural progenitor cells and MSCs detected by loss of MRI signals towards tumor mass and infiltrated tumor cells. Prussian blue staining and fluorescent microscope analysis showed that grafted cells targeted tumor cells and areas with grafted cells corresponded to areas with loss of MRI signals. These results demonstrate that the MRI technique provides a sensitive method for in vivo assessment of grafted cells targeting tumor mass and infiltrated tumor cells and that adult neural progenitor cells and MSCs can target tumor aggregates in the brain. (C) 2004 Elsevier Inc. All rights reserved.