Promotion of cellular migration and apoptosis resistance by a mouse eye-specific phosphatidylethanolamine-binding protein.
Promotion of cellular migration and apoptosis resistance by a mouse eye-specific phosphatidylethanolamine-binding protein.
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DOI：
10.3892/ijmm.19.1.55
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发表时间：
2007
影响因子：
5.4
通讯作者：
Yuanyuan Zhang;Xiaojian Wang;Z. Xiang;Hongzhe Li;J. Qiu;Qiaoling Sun;T. Wan;Nan Li;Xuetao Cao;Jianli Wang
中科院分区：
文献类型：
--
作者：
Yuanyuan Zhang;Xiaojian Wang;Z. Xiang;Hongzhe Li;J. Qiu;Qiaoling Sun;T. Wan;Nan Li;Xuetao Cao;Jianli Wang
磷脂酰乙醇胺 (PE) 结合蛋白 (PEBP) 家族是在各种物种中发现的具有多种功能的进化保守蛋白组。我们鉴定出人类 PE 结合蛋白 4 (hPEBP4)（PEBP 家族的新成员）作为抗凋亡分子。在这里，我们描述了 hPEBP4 小鼠同源物（小鼠 PEBP4、mPEBP4）的克隆和功能表征。 mPEBP4的全长cDNA包含1048bp，开放阅读框(ORF)为729bp，预计编码242个氨基酸的蛋白质。 mPEBP4 包含一个 PE 结合结构域，在本例中位于氨基酸 106 和 213 之间。mPEBP4 主要定位于内质网/高尔基体。有趣的是，RT-PCR和原位杂交分析表明mPEBP4在小鼠眼组织中特异性表达。我们证明mPEBP4通过抑制ERK1/2和JNK激活以及上调COX-2的表达来促进细胞迁移和侵袭。此外，mPEBP4 过表达可抑制表阿霉素诱导的细胞凋亡。考虑到mPEBP4在视网膜神经节细胞中特异性表达，mPEBP4是否是参与视觉功能的重要分子还需要进一步研究。
The phosphatidylethanolamine (PE)-binding protein (PEBP) family is an evolutionary conserved group of proteins found in various species with multiple functions. We identified human PE-binding protein 4 (hPEBP4), a novel member of the PEBP family, as an anti-apoptotic molecule. Here we describe the cloning and functional characterization of the mouse homolog of hPEBP4 (mouse PEBP4, mPEBP4). Full-length cDNA of mPEBP4 contains 1048 bp with an open reading frame (ORF) of 729 bp, which is predicted to encode a 242-aa protein. mPEBP4 contains a PE-binding domain, in this case between amino acids 106 and 213. mPEBP4 localizes primarily to endoplasmic reticulum/Golgi apparatus. Interestingly, RT-PCR and in situ hybridization analyses indicate that mPEBP4 is specifically expressed in mouse eye tissue. We demonstrate that mPEBP4 promotes cellular migration and invasion by inhibiting ERK1/2 and JNK activation and up-regulating the expression of COX-2. In addition, mPEBP4 overexpression inhibits Epirubicin-induced cellular apoptosis. Considering that mPEBP4 is specifically expressed in retinal ganglion cells, whether mPEBP4 is an important molecule involved in visual function needs to be further investigated.