Membrane vesicles in sea water: heterogeneous DNA content and implications for viral abundance estimates
Membrane vesicles in sea water: heterogeneous DNA content and implications for viral abundance estimates
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DOI：
10.1038/ismej.2016.134
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发表时间：
2017-02-01
期刊：
影响因子：
11
通讯作者：
Chisholm, Sallie W.
中科院分区：
文献类型：
--
作者：
Biller, Steven J.;McDaniel, Lauren D.;Chisholm, Sallie W.
不同的微生物从它们的外表面向周围环境释放膜结合的胞外小泡。囊泡存在于包括海洋在内的众多生境中，它们在微生物生态系统中可能具有各种功能。众所周知，细胞外小泡含有包括DNA在内的一系列生物分子，但DNA被包装在小泡中的频率尚不清楚。在这里，我们检查了与五种不同细菌释放的囊泡相关的DNA的数量和分布。囊泡中释放的双链DNA的平均数量和DNA片段的大小分布在不同的类群中是不同的。尽管一些囊泡含有足够的DNA，在SYBR荧光DNA染料染色后可以看到，但这只是一小部分(
Diverse microbes release membrane-bound extracellular vesicles from their outer surfaces into the surrounding environment. Vesicles are found in numerous habitats including the oceans, where they likely have a variety of functional roles in microbial ecosystems. Extracellular vesicles are known to contain a range of biomolecules including DNA, but the frequency with which DNA is packaged in vesicles is unknown. Here, we examine the quantity and distribution of DNA associated with vesicles released from five different bacteria. The average quantity of double-stranded DNA and size distribution of DNA fragments released within vesicles varies among different taxa. Although some vesicles contain sufficient DNA to be visible following staining with the SYBR fluorescent DNA dyes typically used to enumerate viruses, this represents only a small proportion (