Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering
Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering
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DOI：
10.1016/s0142-9612(01)00051-5
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发表时间：
2001-11-01
期刊：
影响因子：
14
通讯作者：
West, JL
中科院分区：
文献类型：
--
作者：
Mann, BK;Gobin, AS;West, JL
可光聚合的聚乙二醇（PEG）衍生物已作为水凝胶组织工程支架被研究。这些材料已用生物活性肽进行修饰，以制造出能模拟天然细胞外基质（ECM）某些特性的材料。其主链中含有可被蛋白酶降解的肽的PEG衍生物已被用于形成可被参与细胞迁移的酶（如胶原酶和弹性蛋白酶）降解的水凝胶。细胞黏附肽，如RGD肽，已被接枝到光聚合水凝胶中以实现生物特异性细胞黏附。在光聚合过程中均匀接种在水凝胶中的细胞保持存活、增殖并产生细胞外基质蛋白。细胞也能在含有可被蛋白酶降解的肽和细胞黏附肽的水凝胶中迁移。通过在光聚合之前制造各种生物活性PEG衍生物的混合物，这些材料的生物活性可被调整以满足特定组织工程应用的要求。（C）2001爱思唯尔科学有限公司。保留所有权利。
Photopolymerizable polyethylene glycol (PEG) derivatives have been investigated as hydrogel tissue engineering scaffolds. These materials have been modified with bioactive peptides in order to create materials that mimic some of the properties of the natural extracellular matrix (ECM). The PEG derivatives with proteolytically degradable peptides in their backbone have been used to form hydrogels that are degraded by enzymes involved in cell migration, such as collagenase and elastase. Cell adhesive peptides, such as the peptide RGD, have been grafted into photo polymerized hydrogels to achieve biospecific cell adhesion. Cells seeded homogeneously in the hydrogels during photopolymerization remain viable, proliferate. and produce ECM proteins. Cells can also migrate through hydrogels that contain both proteolytically degradable and cell adhesive peptides. The biological activities of these materials can be tailored to meet the requirements of a given tissue engineering application by creating a mixture of various bioactive PEG derivatives prior to photopolymerization. (C) 2001 Elsevier Science Ltd. All rights reserved.