QconCAT Standard for Calibration of Ion Mobility-Mass Spectrometry Systems
QconCAT Standard for Calibration of Ion Mobility-Mass Spectrometry Systems
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DOI：
10.1021/pr3005327
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发表时间：
2012-11-01
影响因子：
4.4
通讯作者：
Eyers, Claire E.
中科院分区：
文献类型：
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作者：
Chawner, Ross;McCullough, Bryan;Eyers, Claire E.
离子迁移率-质谱仪(IM-MS)是一种除了测定质量/电荷比外，还能确定分析物离子构象信息的有用技术。控制离子在均匀电场存在下通过气体的迁移率的物理原理是众所周知的，这使得旋转平均碰撞截面(52)能够直接根据在明确定义的实验条件下测量的漂移时间来计算。然而，对于行波(T波)迁移率分离，由于影响离子通过迁移室的一系列因素的影响，这样的“第一原理”计算并不直接。如果T波迁移率分离需要碰撞截面信息，那么使用已知标准对仪器进行校准对于每组实验条件都是必不可少的。为了便于这种校准，我们设计并生产了一种基于QconCAT技术的人造蛋白质QCAL-IM，它在蛋白质分解后可以用作通用的离子迁移率校准标准。这一唯一的标准能够根据T波迁移率仪器上的漂移时间经验计算多肽离子碰撞截面。
Ion mobility-mass spectrometry (IM-MS) is a useful technique for determining information about analyte ion conformation in addition to mass/charge ratio. The physical principles that govern the mobility of an ion through a gas in the presence of a uniform electric field are well understood, enabling rotationally averaged collision cross sections (52) to be directly calculated from measured drift times under well-defined experimental conditions. However, such "first principle" calculations are not straightforward for Traveling Wave (T-Wave) mobility separations due to the range of factors that influence ion motion through the mobility cell. If collision cross section information is required from T-Wave mobility separations, then calibration of the instruments using known standards is essential for each set of experimental conditions. To facilitate such calibration, we have designed and generated an artificial protein based on the QconCAT technology, QCAL-IM, which upon proteolysis can be used as a universal ion mobility calibration standard This single unique standard enables empirical calculation of peptide ion collision cross sections from the drift time on a T-Wave mobility instrument.