Differential efficiency in exogenous DNA acquisition among closely related Salmonella strains: implications in bacterial speciation.
Differential efficiency in exogenous DNA acquisition among closely related Salmonella strains: implications in bacterial speciation.
复制标题
密切相关的沙门氏菌菌株之间外源 DNA 获取的差异效率：对细菌物种形成的影响
DOI：
10.1186/1471-2180-14-157
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发表时间：
2014-06-14
期刊：
影响因子：
4.2
通讯作者：
Liu SL
中科院分区：
文献类型：
--
作者：
Bao HX;Tang L;Yu L;Wang XY;Li Y;Deng X;Li YG;Li A;Zhu DL;Johnston RN;Liu GR;Feng Y;Liu SL
背景
外源遗传物质的获得是细菌物种形成的关键事件。这似乎是合理的假设，重组进入基因组的DNA将是更有效的DNA供体和受体之间的相关性更高的水平。如果是这样的话，细菌物种形成将是一个平稳的过程，导致细菌基因组差异的连续谱，然而，最近的研究结果表明情况并非如此。本研究的目的是确定DNA转移效率是否与序列同一性水平相关。
结果
为了比较密切相关的细菌之间的外源DNA收购的相对效率，我们进行了噬菌体介导的转导和质粒介导的转化在具有不同水平的相关性的代表性沙门氏菌菌株。我们发现，即使在基因几乎相同的细菌之间，效率也有很大差异。虽然有一个普遍的趋势，更密切相关的DNA供体-受体对具有更高的转导效率，转化效率表现出超过一千倍的密切相关的沙门氏菌菌株之间的差异。
结论
在具有高达99%以上相同遗传背景的细菌中，DNA获取效率差异很大，这表明细菌物种形成涉及高度复杂的过程，不仅受环境中是否存在有益的外源DNA的影响，而且还受细菌接受它的“准备程度”的影响。
Background
Acquisition of exogenous genetic material is a key event in bacterial speciation. It seems reasonable to assume that recombination of the incoming DNA into genome would be more efficient with higher levels of relatedness between the DNA donor and recipient. If so, bacterial speciation would be a smooth process, leading to a continuous spectrum of genomic divergence of bacteria, which, however, is not the case as shown by recent findings. The goal of this study was todetermine if DNA transfer efficiency is correlated with the levels of sequence identity.
Results
To compare the relative efficiency of exogenous DNA acquisition among closely related bacteria, we carried out phage-mediated transduction and plasmid-mediated transformation in representative Salmonella strains with different levels of relatedness. We found that the efficiency was remarkably variable even among genetically almost identical bacteria. Although there was a general tendency that more closely related DNA donor-recipient pairs had higher transduction efficiency, transformation efficiency exhibited over a thousand times difference among the closely related Salmonella strains.
Conclusion
DNA acquisition efficiency is greatly variable among bacteria that have as high as over 99% identical genetic background, suggesting that bacterial speciation involves highly complex processes affected not only by whether beneficial exogenous DNA may exist in the environment but also the “readiness” of the bacteria to accept it.
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DOI：
10.1073/pnas.93.19.10303
发表时间：
1996-09-17
影响因子：
11.1
作者：
Liu, SL;Sanderson, KE
通讯作者：
Sanderson, KE
影响因子：
4
作者：
OCHMAN, H;BERGTHORSSON, U
通讯作者：
BERGTHORSSON, U
影响因子：
11.4
作者：
GROISMAN, EA;SAIER, MH;OCHMAN, H
通讯作者：
OCHMAN, H
影响因子：
3.7
作者：
Feng Y;Johnston RN;Liu GR;Liu SL
通讯作者：
Liu SL
DOI：
10.1073/pnas.92.4.1018
发表时间：
1995-02-14
影响因子：
11.1
作者：
LIU, SL;SANDERSON, KE
通讯作者：
SANDERSON, KE