Sculpting Enzyme-Generated Giant Polymer Brushes
Sculpting Enzyme-Generated Giant Polymer Brushes
复制标题
雕刻酶生成的巨型聚合物刷
DOI：
10.1021/acsnano.0c06882
复制
发表时间：
2021
期刊：
影响因子：
17.1
通讯作者：
Curtis, Jennifer E.
中科院分区：
文献类型：
--
作者：
Faubel, Jessica L.;Wei, Wenbin;Curtis, Jennifer E.
我们提出了一种简单而通用的方法来雕刻超厚的，酶生成的透明质酸聚合物刷子与光。图案化机制是间接的，由与底层衬底的光化学作用产生的活性氧物种驱动。活性氧会破坏透明质酸合成酶，而透明质酸合成酶是末端接枝聚合物的生长引擎和锚。在刷子聚合之前或之后，通过以能量密度剂量依赖的方式使酶失活来实现对接枝密度的空间控制。使用可见波长可以定量改变刷子高度，并且通过在长度为56μm(大约每微米增加90 nm高度)上创建高度范围为0到6μm的刷子梯度来说明。在这项研究中提出的基本见解的基础上，这项工作为用酶生成的透明质酸画笔灵活和定量地雕刻复杂的三维景观奠定了基础。
We present a simple yet versatile method for sculpting ultra-thick, enzyme-generated hyaluronan polymer brushes with light. The patterning mechanism is indirect, driven by reactive oxygen species created by photochemical interactions with the underlying substrate. The reactive oxygen species disrupt the enzyme hyaluronan synthase, which acts as the growth engine and anchor of the end-grafted polymers. Spatial control over the grafting density is achieved through inactivation of the enzyme in an energy density dose-dependent manner, before or after polymerization of the brush. Quantitative variation of the brush height is possible using visible wavelengths and illustrated by the creation of a brush gradient ranging from 0 to 6 μm in height over a length of 56 μm (approximately a 90 nm height increase per micron). Building upon the fundamental insights presented in this study, this work lays the foundation for the flexible and quantitative sculpting of complex three-dimensional landscapes in enzyme-generated hyaluronan brushes.