Microbiota of health, gingivitis, and initial periodontitis
Microbiota of health, gingivitis, and initial periodontitis
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DOI：
10.1111/j.1600-051x.1998.tb02414.x
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发表时间：
1998-02-01
影响因子：
6.7
通讯作者：
Kent, RL
中科院分区：
文献类型：
--
作者：
Tanner, A;Maiden, MFJ;Kent, RL
本研究采用优势培养法、DNA探针法、棋盘杂交法比较牙周健康、牙龈炎和初发牙周炎患者的龈下微生物群。对56例牙周附着物轻度丧失的健康成人进行临床监测，每隔3个月进行一次，持续12个月。在监测期间，更多的地点表现出依恋损失的小增量，而不是依恋获得的小增量。在监测过程中，从17名受试者中选出大于或等于1.5 mm牙周附着物损失的部位作为活动性病变进行微生物分析。12名受试者表现为近端间病变，5名受试者在颊部部位出现附着丧失（衰退）。培养研究发现，连翘拟杆菌、直弯曲杆菌和恶硒单胞菌是与活跃的近端间病变相关的优势菌种（9名受试者），而naeslundii放线菌和口腔链球菌是定殖在口腔活跃部位的优势菌种。10名受试者牙龈炎培养的优势菌种为A. naeslundii、弯曲杆菌（Campylobacter gracilis）和连翘杆菌（B. forsythus）（水平低于活性位点）。与健康相关的物种（10名受试者）包括口腔链球菌、纳氏单胞菌和放线菌。DNA探针数据显示，与不活跃牙周炎部位相比，活跃牙周炎部位（7名受试者）的连翘芽孢杆菌和直直芽孢杆菌的平均水平较高。牙龈卟啉单胞菌和放线菌comitans检出较少。培养菌群聚类分析将8/9个活跃的近端间病变归为一个亚群，其特征主要是革兰氏阴性菌群，包括连翘芽孢杆菌和直叶芽孢杆菌。结果表明，连翘B. forsythus C. rectus和noxia S. noxia是牙周健康向疾病转化的主要物种。近端间和颊部活性部位的位置和微生物群的差异表明，不同的机制可能参与了附着丧失的增加。
This study compared the subgingival microbiota in periodontal health, gingivitis and initial periodontitis using predominant culture and a DNA probe, checkerboard hybridization method. 56 healthy adult subjects with minimal periodontal attachment loss were clinically monitored at 3-month intervals for 12 months. More sites demonstrated small increments of attachment loss than attachment gain over the monitoring period. Sites, from 17 subjects, showing greater than or equal to 1.5 mm periodontal attachment loss during monitoring were sampled as active lesions for microbial analysis. Twelve subjects demonstrated interproximal lesions, and 5 subjects had attachment loss at buccal sites (recession). Cultural studies identified Bacteroides forsythus, Campylobacter rectus, and Selenomonas noxia as the predominant species associated with active interproximal lesions (9 subjects), whereas Actinomyces naeslundii, and Streptococcus oralis, were the dominant species colonizing buccal active sites. A. naeslundii, Campylobacter gracilis, and B. forsythus (at lower levels than active sites) were the dominant species cultured from gingivitis (10 subjects). Health-associated species (10 subjects) included Streptococcus oralis, A. naeslundii, and Actinomyces gerencseriae. DNA probe data identified higher mean levels of B. forsythus and C. rectus with active (7 subjects) compared to inactive periodontitis sites. Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were detected infrequently. Cluster analysis of the cultural microbiota grouped 8/9 active interproximal lesions in one subcluster characterized by a mostly gram-negative microbiota, including B. forsythus and C. rectus. The data suggest that B. forsythus C. rectus and S. noxia were major species characterizing sites converting from periodontal health to disease. The differences in location and microbiota of interproximal and buccal active sites suggested that different mechanisms may be involved in increased attachment loss.