Soft-matrices based on silk fibroin and alginate for tissue engineering
Soft-matrices based on silk fibroin and alginate for tissue engineering
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DOI：
10.1016/j.ijbiomac.2016.04.045
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发表时间：
2016-12-01
影响因子：
8.2
通讯作者：
Boccaccini, Aldo R.
中科院分区：
文献类型：
--
作者：
Silva, Raquel;Singh, Raminder;Boccaccini, Aldo R.
软组织再生需要使用具有足够机械性能以及提供营养和氧气以及去除代谢生物产物的能力的基质。在这项工作中，我们描述了基于海藻酸盐（Alg）和丝素蛋白（SF）混合物的水凝胶的开发。在此，我们报告了将细胞与生物材料结合的两种主要策略：细胞要么被接种到预制水凝胶薄膜上（2D），要么在水凝胶微胶囊形成过程中被封装（3D）。两种几何形状均已成功生产并表征。 FTIR 结果表明，水凝胶形成后，SF 的构象从无规卷曲变为 β 片层。由 Alg 和 Alg/SF 制成的薄膜和微胶囊的热降解行为取决于水凝胶组成和样品的几何形状。 SF的存在导致吸水能力下降并影响降解行为。机械测试表明，添加 SF 会促进储能模量的增加，从而使材料比纯 Alg 更硬（刚度高 6 倍）。此外，使用人脐静脉内皮细胞（HUVEC）研究了不同几何形状的 Alg/SF 水凝胶的体外细胞-材料相互作用。与纯 Alg 相比，Alg/SF 水凝胶上 HUVEC 的活力、附着、扩散和增殖显着增加。这些发现表明 Alg/SF 水凝胶是一种在组织工程和再生生物医学应用中很有前景的材料。 (C) 2016 Elsevier B.V. 保留所有权利。
Soft tissue regeneration requires the use of matrices that exhibit adequate mechanical properties as well as the ability to supply nutrients and oxygen, and to remove metabolic bio-products. In this work, we describe the development of hydrogels based on the blend between alginate (Alg) and silk fibroin (SF). Herein, we report two main strategies to combine cells with biomaterials: cells are either seeded onto prefabricated hydrogels films (2D), or encapsulated during hydrogel microcapsules formation (3D). Both geometries were successfully produced and characterized. FTIR results indicated a change of conformation of SF from random coil to beta-sheet after hydrogel formation. The thermal degradation behavior of films and microcapsules fabricated from Alg, and Alg/SF was dependent on the hydrogel composition and on the geometry of the samples. The presence of SF caused decreased water uptake ability and affected the degradation behavior. Mechanical tests showed that addition of SF promotes an increase in storage modulus, leading to a stiffer material as compared with pure Alg (6 times higher stiffness). Moreover, the in vitro cell-material interaction on Alg/SF hydrogels of different geometries was investigated using human umbilical vein endothelial cells (HUVECs). Viability, attachment, spreading and proliferation of HUVECs were significantly increased on Alg/SF hydrogels compared to neat Alg. These findings indicate that Alg/SF hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration. (C) 2016 Elsevier B.V. All rights reserved.