Hot RAD: A Tool for Analysis of Next-Gen RAD Tag Data
Hot RAD: A Tool for Analysis of Next-Gen RAD Tag Data
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Hot RAD：下一代 RAD 标签数据分析工具
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
S. Emrich
中科院分区：
文献类型：
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作者：
Lauren A. Assour;Nicholas LaRosa;S. Emrich
限制性内切位点相关DNA （RAD）标记（也称为RAD-seq等）是一种新兴的不需要完全测序就能分析生物体基因组的方法。这可以应用于没有参考基因组的非模式生物，尽管这会产生如何在未映射和未注释的读取上开始数据分析的问题。我们的程序Hot RAD提供了一种简单易用的方法，可以获取已被RAD标记的原始Illumina数据，并使用分布式框架生成共识组或序列堆栈，从而为开始分析生物体的DNA奠定基础。我们的工具的GUI（图形用户界面）元素使那些不熟悉命令行的人可以很容易地获取原始序列文件并及时生成可用的数据。
Restriction site Associated DNA (RAD) tagging (also known as RAD-seq, etc.) is an emerging method for analyzing an organism's genome without completely sequencing it. This can be applied to a non-model organism without a reference genome, though this creates the problem of how to begin data analysis on unmapped and unannotated reads. Our program, Hot RAD, presents a straightforward and easy-to-use method to take raw Illumina data that has been RAD tagged and produce consensus contigs or sequence stacks using a distributed framework, creating a basis on which to begin analyzing an organism's DNA. The GUI (graphical user interface) element of our tool makes it easy for those not familiar with the command line to take raw sequence files and produce usable data in a timely manner.