The sexually dimorphic nucleus of the hypothalamus contains GABA neurons in rat and man
The sexually dimorphic nucleus of the hypothalamus contains GABA neurons in rat and man
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DOI：
10.1016/s0006-8993(96)01005-0
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发表时间：
1996-12-02
期刊：
影响因子：
2.9
通讯作者：
Moore, RY
中科院分区：
文献类型：
--
作者：
Gao, B;Moore, RY
视前区性别二态性核（SDN-POA）是大鼠大脑中表现出形态性别差异的最引人注目的结构。在人类下丘脑中发现了一个潜在的同源核。本研究的目的是通过确定大鼠和人类 SDN-POA 是否表达相同的递质表型来追求它们的推定同源性。我们采用 GAD mRNA 原位杂交组织化学来显示 SDN-POA 的神经元是否产生 GABA。在大鼠和人类中，大多数（如果不是全部）SDN-POA 神经元中都存在高水平的 GAD(65) 和 GAD(67) mRNA。大鼠或人类的表达水平没有明显的性别差异。数据表明，大鼠和人类的 SDN-POA 神经元均产生 GABA，并支持大鼠和人类下丘脑中这些细胞核的同源性。
The sexually dimorphic nucleus of the preoptic area (SDN-POA) is the most striking structure displaying a morphological sex difference in the rat brain. A potentially homologous nucleus has been identified in the human hypothalamus. The objective of the present study was to pursue the putative homology of the rat and human SDN-POA by determining whether they express the same transmitter phenotype. We employed in situ hybridization histochemistry for GAD mRNA to show whether the neurons of the SDN-POA produce GABA. In both the rat and human, high levels of GAD(65) and GAD(67) mRNA are present in most, if not all, SDN-POA neurons. No sex difference is evident in the level of expression in either the rat or human. The data indicate that neurons of the SDN-POA in both the rat and human are GABA-producing and argue for the homology of these nuclei in the rat and human hypothalamus.