Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing
Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing
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DOI：
10.1073/pnas.96.24.13789
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发表时间：
1999-11-23
影响因子：
11.1
通讯作者：
Gould, KL
中科院分区：
文献类型：
--
作者：
Burns, CG;Ohi, R;Gould, KL
Myb相关蛋白的保守的CDC 5家族在G（2）/M期的细胞周期控制中发挥重要作用。虽然c-Myb和许多Myb相关蛋白作为转录因子，在这里，我们牵连CDC 5蛋白在前mRNA剪接。哺乳动物CDC 5与哺乳动物细胞核中的前mRNA剪接因子共定位，与核提取物中剪接机制的核心组分相关联，并在体外整个剪接反应中与剪接体相互作用。此外，在酿酒酵母（Saccharomyces cerevisiae，CEF 1.阻断了体内前体mRNA加工的第一步。这些数据提供了真核细胞需要CDC 5蛋白进行前mRNA剪接的证据。
The conserved CDC5 family of Myb-related proteins performs an essential function in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei of mammalian cells, associates with core components of the splicing machinery in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1. blocks the first step of pre-mRNA processing in vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for pre-mRNA splicing.