Sparstolonin B inhibits lipopolysaccharide-induced inflammation in 3T3-L1 adipocytes
Sparstolonin B inhibits lipopolysaccharide-induced inflammation in 3T3-L1 adipocytes
复制标题
Sparstolonin B 抑制 3T3-L1 脂肪细胞中脂多糖诱导的炎症。
DOI：
10.1016/j.ejphar.2015.10.050
复制
发表时间：
2015-12-15
影响因子：
5
通讯作者：
Sun, Jia
中科院分区：
文献类型：
--
作者：
Wang, Ming;Xiu, Liangchang;Sun, Jia
玄参甲素B(SSnB)是从三棱和三棱中分离得到的一种异香豆素类化合物，具有抗炎作用。然而，SSNB对内毒素刺激的3T3-L1脂肪细胞是否具有抗炎作用尚不清楚。在本研究中，我们观察了SSnB对高脂饮食(HFD)诱导的肥胖大鼠3T3-L1脂肪细胞炎症和抗肥胖特性的影响。3T3-L1脂肪细胞在脂多糖处理前1h用SSNB进行预处理。采用定量逆转录聚合酶链式反应和酶联免疫吸附试验检测MCP-1、IL-6、TNIF-α和IL-10的表达。免疫印迹法检测PPAR-γ、TLR4和核因子-kappaB的表达。对HFD诱导的肥胖大鼠给予丹参皂苷B，以证实其体内作用。结果表明，丹参B剂量依赖性地抑制内毒素诱导的单核细胞趋化蛋白-1、IL-6和肿瘤坏死因子-α的产生。SSNB可抑制LPS诱导的TLR4表达和核因子-kappaB的激活。PPAR-γ拮抗剂GW9662可逆转SSnB对MCP-1、IL-6和TNIF-α产生的抑制作用。在体内，SSNB被发现可以减轻高脂饲料喂养的大鼠的体重。丹参B还能抑制高脂饲料诱导的小鼠血清甘油三酯(TG)和胆固醇(TC)水平。综上所述，SSNB可通过激活PPAR-γ抑制脂多糖诱导的3T3-L1脂肪细胞产生细胞因子，从而减轻HFD诱导的大鼠肥胖。(C)2015爱思唯尔B.V.保留所有权利。
Sparstolonin B (SsnB), an isocoumarin compound isolated from the tubers of both Sparganium stoloniferum and Scirpus yagara, has been reported to have anti-inflammatory effects. However, whether SsnB has anti-inflammatory effects on LPS-stimulated 3T3-L1 adipocytes remains unclear. In this study, we investigated the effects of SsnB on adipocyte inflammation in 3T3-L1 adipocytes and anti-obesity properties in high fat diet (HFD)-induced obese rats. 3T3-L1 adipocytes were pretreated with SsnB 1 h before LPS treatment. The expression of MCP-1, IL-6, TNIF-alpha, and IL-10 were measured by qRT-PCR and ELISA. The expression of PPAR-gamma, TLR4 and NF-kappa B were detected by western blotting. SsnB was administered to HFD-induced obese rats to confirm its effects in vivo. Our results showed that SsnB dosedependently inhibited LPS-induced MCP-1, IL-6, and TNIF-alpha production. SsnB was found to inhibit LPSinduced TLR4 expression and NF-kappa B activition. Furthermore, SsnB was found to activate PPAR-gamma and the inhibitory effects of SsnB on MCP-1, IL-6, and TNIF-alpha production can be reversed by PPAR-gamma antagonist GW9662. In vivo, SsnB was found to reduce the body weight of rats fed with HFD. SsnB also inhibited the levels of serum triglyceride (TG) and cholesterol (TC) induced by HFD. In conclusion, the results suggested that SsnB could reduce HFD-induced obesity in rats and inhibited LPS-induced cytokines production in 3T3-L1 adipocytes by activating PPAR-gamma. (C) 2015 Elsevier B.V. All rights reserved.